Anti-GMFB Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P60983

Core Product Specifications and Parameters

Biological Background: Glia maturation factor beta Function and Localization

• Glia maturation factor beta (GMFB) is a brain-derived growth factor known for its role in glial cell differentiation and neural regeneration.
• It promotes the differentiation of brain cells, supporting neural development and repair processes.
• GMFB stimulates neural regeneration, potentially aiding recovery after neuronal injury.
• The protein exhibits tumor suppressor-like activity by inhibiting the proliferation of tumor cells.
• GMFB is a member of the GMF family and is annotated as a growth factor, highlighting its role in cellular growth and differentiation.
• Post-translational modifications include phosphorylation and acetylation, which may regulate its activity.
• Identified in high-throughput proteomics studies, GMFB is part of the reference proteome for human.

Experimental Guidance and Technical Tips

• This antibody is raised against the full-length human GMFB protein (aa 1-140), which may recognize the native conformation as well as denatured forms.
• For Western blotting, consider using whole-cell lysates from brain or neural cell lines; the predicted molecular weight is ~17 kDa, so optimize gel percentage and transfer conditions accordingly.
• For immunocytochemistry/immunofluorescence, fixation and permeabilization conditions should be optimized for the cell type and localization (note that specific subcellular localization data is not provided, so validation may be required).
• Since the antibody cross-reacts with mouse and rat, it can be used for comparative studies across these species; validate in relevant tissue samples.
• ELISA protocols may be performed using the recombinant protein as a standard; consider coating with the immunogen or fragment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting. This anti-GMFB antibody supports research in neuroscience and oncology, and CamelBio offers validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for diagnostic development. For rare target sourcing or custom requirements, our team bridges the gap from concept to clinic.