Anti-Gm13125/PRAMEF20 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q5VT98

Core Product Specifications and Parameters

Biological Background: PRAMEF20 (Gm13125) Function and Localization

• PRAMEF20 (Preferentially Expressed Antigen in Melanoma Family Member 20) is a protein characterized by leucine-rich repeats (LRRs), which are often involved in protein-protein interactions.
• The gene is encoded by PRAMEF20 (also designated PRAMEF21) in humans and belongs to the PRAME family.
• The protein has a calculated molecular mass of approximately 54.8 kDa, consistent with the detected band around 55 kDa.
• LRR motifs are commonly found in proteins participating in signal transduction, cell adhesion, and immune responses.
• It is catalogued as part of the reference proteome, indicating comprehensive annotation in curated databases.
• Currently, no specific subcellular localization or detailed function has been experimentally established for this protein.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 67–274 of mouse Gm13125, which includes predicted LRR regions; this conservation may underlie the observed cross-reactivity with human and rat.
• For Western blot (WB), consider a starting dilution of 1:500–1:2000 and validate with lysates from mouse, human, and rat cell lines as positive controls.
• For immunohistochemistry (IHC-P), antigen retrieval steps may be required; always include matched tissue controls and, if possible, verify staining by blocking with the immunizing peptide or using knockdown samples.
• For ELISA, conditions should be optimized with recombinant protein or cell extracts; check coating buffers and detection parameters for optimal signal-to-noise ratio.
• As the protein’s intracellular localization remains uncharacterized, it is advisable to examine both nuclear and cytoplasmic fractions during initial validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. For targets like PRAMEF20 (Gm13125), we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare target raw materials. Let us streamline your development pipeline with reliable, high-quality immunological tools.