Anti-GM130 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q08379

Core Product Specifications and Parameters

Biological Background: GM130 Function and Localization

• GM130 (GOLGA2), also referred to as 130 kDa cis-Golgi matrix protein, Golgin-95, or GM130 autoantigen, is a peripheral membrane component of the cis-Golgi stack that serves as a membrane skeleton to maintain Golgi architecture.
  Related references: PMID:16489344

• Together with p115/USO1 and STX5, GM130 mediates vesicle tethering and fusion at the cis-Golgi membrane, critical for maintaining the stacked and interconnected Golgi structure.

• GM130 is essential for normal ER-to-Golgi and secretory protein transport.

• Mitotic phosphorylation of GM130 at Ser-37 by CDK1 inhibits its interaction with p115/USO1, blocking COPI vesicle tethering and triggering Golgi disassembly during cell division.

• GM130 promotes mitotic spindle pole assembly by recruiting importin-alpha to the Golgi, releasing TPX2 to activate AURKA and nucleate microtubules.
  Related references: PMID:19242490 PMID:26165940

• GM130 is required for Golgi ribbon formation and proper glycosylation of membrane and secretory proteins.
  Related references: PMID:16489344 PMID:17314401

• GM130 also plays a role in centrosome organization.
  Related references: PMID:18045989 PMID:19109421

• Subcellularly, GM130 localizes primarily to the cis-Golgi network membrane, ER-Golgi intermediate compartment, and mitotic spindle poles.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 20-300) of GM130, which includes domains involved in Golgi targeting and protein interactions; verify epitope accessibility under your specific fixation and permeabilization conditions for IF/ICC and IHC-P applications.
• For western blotting, the predicted molecular weight of GM130 is ~113 kDa; use appropriate gel percentage (e.g., 8% SDS-PAGE) and extended transfer times for high-molecular-weight proteins.
• Cross-reactivity with mouse and rat GM130 has been confirmed; if using other species, perform sequence alignment and validation in the relevant sample system.
• As a cis-Golgi marker, GM130 is commonly used in immunofluorescence; consider co-staining with markers of other organelles (e.g., GM130 for cis-Golgi, TGN46 for trans-Golgi) to assess Golgi morphology and function.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality IVD raw materials, including validated GM130 antibodies for Golgi biology and cell cycle research. Our comprehensive portfolio includes antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents to streamline your assay development and supply chain. Contact us for bulk orders or custom sourcing of rare-target raw materials.