Anti-GM-CSF Rabbit Monoclonal Antibody for WB, ELISA - P48750

Core Product Specifications and Parameters

Biological Background: GM-CSF Function and Localization

• GM-CSF, encoded by the Csf2 gene in rat, is also referred to as Colony-stimulating factor (CSF). It functions as a secreted cytokine.
• The primary role of GM-CSF is to stimulate the growth and differentiation of hematopoietic precursor cells from multiple lineages, including granulocytes, macrophages, eosinophils, and erythrocytes.
• As a glycoprotein with a signal peptide and disulfide bonds, GM-CSF is classified as a growth factor integral to hematopoiesis and immune response regulation.
• GM-CSF is predominantly secreted and acts in the extracellular environment to exert its biological effects on target cells.
• The mature protein has a predicted molecular weight of approximately 16.5 kDa, though glycosylation may result in an apparent mass of around 17 kDa in Western blotting.
• Structural features include disulfide bonds crucial for proper folding and biological activity, consistent with its cytokine nature.
• Keywords associated with GM-CSF include Cytokine, Disulfide bond, Glycoprotein, Growth factor, Reference proteome, Secreted, and Signal, reflecting its function and post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 20–144 of rat GM-CSF, covering the mature secreted form. This suggests the antibody is likely to recognize the biologically active, processed protein.
• For Western blot, consider using reducing conditions to account for disulfide bonds. A band around 17 kDa is expected, though glycosylation may cause slight shifts.
• For ELISA applications, pairing with a detection antibody that binds a non-overlapping epitope may improve assay sensitivity. Validate in appropriate sample types, such as rat serum or cell culture supernatants.
• As a secreted protein, GM-CSF may be present at low concentrations in some samples. Enrichment or concentration steps could be considered to enhance detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio enables diagnostic manufacturers and researchers to source high-quality IVD raw materials efficiently, including validated anti-cytokine monoclonal antibodies like this anti-GM-CSF clone for hematopoiesis and immunoassay development. Beyond individual antibodies, we provide optimized antibody pairs, bulk ancillary reagents, and support for rare target sourcing—ensuring a seamless supply chain from concept to clinical application. Partner with CamelBio for reliable access to critical raw materials and expert technical guidance in biomarker research.