Anti-GM-CSF Monoclonal Antibody for WB, IF/ICC, ELISA - P01587

Core Product Specifications and Parameters

Biological Background: GM-CSF Function and Localization

• GM-CSF (Granulocyte-macrophage colony-stimulating factor) is encoded by the Csf2 gene and also referred to as colony-stimulating factor (CSF).
• It functions as a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils, and erythrocytes.
• Subcellular localization is exclusively secreted, consistent with its role as an extracellular signaling molecule.
• Key structural features include a signal peptide (cleaved to yield the mature 18-141 form) and the presence of disulfide bonds and glycosylation sites, classifying it as a glycoprotein growth factor.
• According to UniProt annotation, the mature protein has a calculated molecular weight of ~16 kDa, aligning with the detected band size.
• Additional keywords highlight its characterization as a cytokine, growth factor, and reference proteome member, with direct protein sequencing evidence.

Experimental Guidance and Technical Tips

• The immunogen spans the mature secreted form (aa 18-141) of mouse GM-CSF, making this antibody well-suited for detecting the endogenous protein in mouse samples.
• For Western blot (WB), validate with appropriate positive controls (e.g., activated T cells or macrophages) and consider the predicted molecular weight of approximately 16 kDa; reducing conditions may be required.
• In immunofluorescence (IF/ICC), note the secreted nature of GM-CSF—optimize permeabilization and fixation protocols to capture intracellular staining, and include a negative control to distinguish specific signal.
• For ELISA applications, assess the antibody’s performance in sandwich or indirect formats with recombinant GM-CSF standards, and determine optimal coating and detection concentrations empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined sourcing solution for high-quality IVD raw materials, including this validated anti-GM-CSF rabbit monoclonal antibody. Supporting cytokine research and immunoassay development, we provide bulk monoclonal/polyclonal antibodies, matched antibody pairs, and ancillary reagents to accelerate your workflow. With technical consulting from concept to clinic, CamelBio simplifies procurement of even rare-target raw materials—helping you move from discovery to diagnostic application efficiently.