Anti-Glypican 3 (GPC3) Monoclonal Antibody for WB, IF/ICC, FC, ELISA - P51654

Core Product Specifications and Parameters

Biological Background: Glypican 3 Function and Localization

• GPC3 is a cell surface proteoglycan that plays diverse roles in signaling and development. Related references: PMID:14610063
• It negatively regulates hedgehog signaling by competing with PTC1 for hedgehog binding; SHH binding triggers internalization and lysosomal degradation (By similarity).
• GPC3 positively regulates canonical Wnt signaling by binding Frizzled and facilitating Wnt-Frizzled interaction. Related references: PMID:16227623, PMID:24496449
• It inhibits the dipeptidyl peptidase activity of DPP4. Related references: PMID:17549790
• GPC3 is involved in limb patterning, skeletal development, renal branching morphogenesis, coronary vascular development, and gastrulation cell movements (By similarity).
• The protein is GPI-anchored to the cell membrane and highly expressed in lung, liver, kidney, and placenta (detected at protein level). Related references: PMID:32337544
• Key post-translational modifications include heparan sulfate attachment, disulfide bonds, and phosphorylation; multiple spliced isoforms exist.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from the C-terminal region (amino acids 481–580) of human GPC3, ensuring epitope specificity.
• Predicted molecular weight is 66 kDa; observed bands may shift due to glycosylation. Validate in your sample system with appropriate controls.
• Suitable for WB, IF/ICC, FC, and ELISA. Optimize antibody dilution and conditions for your specific assay.
• This antibody is validated for human GPC3; cross-reactivity with other species has not been tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized provider of IVD raw materials, offering a comprehensive portfolio from validated antibody pairs to bulk ancillary reagents. This anti-Glypican 3 (GPC3) rabbit monoclonal antibody supports research in cancer biology, developmental signaling, and biomarker discovery. With expertise in rare target sourcing and custom reagent development, CamelBio helps diagnostic manufacturers and laboratories accelerate their projects from concept to clinic.