Anti-Glypican 3 (GPC3) Monoclonal Antibody for Flow Cytometry - P51654

Core Product Specifications and Parameters

Biological Background: Glypican-3 Function and Localization

• Cell surface proteoglycan that negatively regulates the hedgehog signaling pathway by competing with PTC1 for binding to hedgehog proteins; binding to SHH triggers endocytosis and lysosomal degradation. Related references: PMID:14610063

• Positive regulator of the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled, facilitating interaction with Wnt ligands. Related references: PMID:16227623 PMID:24496449

• Inhibits the dipeptidyl peptidase activity of DPP4, modulating growth factor signaling. Related references: PMID:17549790

• Attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor; released extracellularly by lipases.

• Highly expressed in lung, liver, and kidney; detected at the protein level in placenta.

• Contains heparan sulfate and chondroitin sulfate glycosaminoglycan chains; undergoes phosphorylation and disulfide bond formation; subject to alternative splicing.

• Plays essential roles in limb patterning, skeletal development, renal branching morphogenesis, and coronary vascular development.

• Belongs to the glypican family; associated with Simpson-Golabi-Behmel syndrome due to loss-of-function mutations.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 481–580, located in the extracellular domain, suggesting recognition of the native surface protein without permeabilization.

• As a direct conjugate (), the antibody is suitable for flow cytometry; titrate using a known positive cell line (e.g., HepG2) and include an appropriate isotype control.

• Cell surface staining is expected; for intracellular detection, validate fixation/permeabilization protocols as they may affect epitope availability.

• is a bright, photostable dye compatible with standard flow cytometers; minimize light exposure during handling.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with seamless access to premium IVD raw materials, including this monoclonal antibody targeting Glypican 3 (GPC3)—a key biomarker in hepatocellular carcinoma and developmental disorders. As a dedicated B2B partner, CamelBio sources validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials, streamlining your journey from concept to clinic.