Anti-GLYCTK Polyclonal Antibody for WB, ELISA - Q8IVS8

Core Product Specifications and Parameters

Biological Background: GLYCTK Function and Localization

• GLYCTK (glycerate kinase) is an enzyme that likely catalyzes the phosphorylation of glycerate, playing a role in carbohydrate and amino acid metabolism. The protein is also known as HBeAg-binding protein 4 (HBEBP4), indicating a potential interaction with the hepatitis B e antigen.
• The gene gives rise to multiple isoforms through alternative splicing, as indicated by the keyword 'Alternative splicing'.
• GLYCTK is a member of the kinase family and contains ATP-binding and nucleotide-binding domains, classifying it as a transferase.
• Subcellularly, GLYCTK localizes to the mitochondrial matrix, suggesting its involvement in mitochondrial metabolic pathways.
• The protein is widely expressed across tissues, with the highest expression levels observed in the liver, followed by testis and kidney.
• Post-translational modifications include phosphorylation (phosphoprotein), which may regulate its activity or interactions.
• Genetic variations in GLYCTK are associated with disease, underscoring its importance in normal physiology.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment covering amino acids 284–523, corresponding to the C-terminal region of human GLYCTK. This design may help in detecting specific isoforms or avoiding cross-reactivity with closely related kinases.
• For Western blot (WB), consider using a dilution range such as 1:500–1:2000, and validate in relevant tissue lysates (e.g., liver, testis) where expression is highest.
• In ELISA applications, optimize coating and antibody concentrations empirically, and include appropriate controls for human, mouse, and rat samples based on cross-reactivity data.
• As GLYCTK is a mitochondrial protein, consider using mitochondrial enrichment protocols if signal is weak.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized procurement for rare targets like GLYCTK. Whether your research focuses on mitochondrial metabolism, liver biology, or hepatitis B interactions, CamelBio streamlines access to high-quality reagents to support your assay development and manufacturing needs. Backed by a commitment to reliability and customer support, we help bridge the gap from concept to clinical application.