Anti-Glycophorin C (GYPC) Monoclonal Antibody for WB, IHC-P, ELISA - P04921

Core Product Specifications and Parameters

Biological Background: Glycophorin C Function and Localization

• Glycophorin C (GYPC) is a minor sialoglycoprotein expressed on human erythrocyte membranes.
• It carries the Gerbich blood group antigens and acts as a receptor for Plasmodium falciparum merozoites, facilitating malaria invasion.
• The protein plays a critical role in maintaining red blood cell mechanical stability and shape.
• GYPC localizes to the cell membrane with a single-pass transmembrane domain and an extracellular N-terminus.
• Alternative splicing produces isoforms including Glycophorin-D (GPD) and IsoGPC, with GYPC primarily in erythrocytes while others show ubiquitous expression.
• Post-translational modifications include sialylation and phosphorylation, contributing to its structural diversity.
• Keywords from UniProt: 3D-structure, Blood group antigen, Alternative splicing, Glycoprotein, Membrane, Phosphoprotein, Reference proteome, Sialic acid, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 49-128 of human GYPC; consider validating cross-reactivity in your specific mouse or rat sample systems.
• For Western blot, expect a band near 14 kDa under reducing conditions; optimize loading and transfer conditions for low-molecular-weight targets.
• In IHC-P, standard antigen retrieval protocols and appropriate controls are recommended to confirm specificity in tissue sections.
• For ELISA, perform checkerboard titrations to determine optimal coating and antibody concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined pathway to secure validated IVD raw materials. This anti-Glycophorin C monoclonal antibody supports red blood cell membrane and malaria infection research, exemplifying our commitment to delivering high-quality antibodies and ancillary reagents. Explore our catalog for bulk options, customized panels, and expert technical support to accelerate your diagnostic development.