Anti-Glycogen synthase 1 (GYS1) Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P13807

Core Product Specifications and Parameters

Biological Background: Glycogen synthase 1 (GYS1) Function and Localization

• Glycogen synthase 1 (GYS1), also known as glycogen synthase muscle isoform, is a key enzyme in glycogen biosynthesis, encoded by the GYS1 gene.
• GYS1 catalyzes the rate-limiting step in glycogen synthesis by transferring the glycosyl residue from UDP-glucose to the non-reducing end of an alpha-1,4-glucan primer, extending the glycogen chain.
• This enzyme works in concert with glycogenin (which initiates the primer) and glycogen branching enzyme to form mature glycogen particles.
• GYS1 is highly expressed in skeletal muscle and is also present in most cell types where glycogen storage occurs.
• The activity of GYS1 is tightly regulated by allosteric effectors and reversible phosphorylation, linking glycogen metabolism to insulin signaling and energy status.
• Alternative splicing of GYS1 produces multiple transcript variants, which may contribute to tissue-specific regulation of glycogen synthesis.
• Mutations in GYS1 are associated with diabetes mellitus and glycogen storage disorders, underscoring its critical role in glucose homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal fragment (aa 488-737) of human GYS1, which shares high homology with mouse and rat GYS1; verify cross-reactivity in your sample system if using non-human species.
• For Western blotting, the predicted molecular weight of GYS1 is ~84 kDa; use appropriate protein ladders and optimize loading controls for glycogen-rich tissues.
• In immunohistochemistry (IHC-P), consider using skeletal muscle or liver sections as positive controls, and optimize antigen retrieval conditions.
• For immunofluorescence (IF/ICC), permeabilization may be required to detect this cytoplasmic enzyme; validate subcellular localization under various metabolic conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with reliable raw materials for metabolic and diabetes-related biomarker development. We offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for glycogen metabolism research. For rare target sourcing, our team provides customized solutions to accelerate your assay development from concept to clinic.