Anti-Glycerol kinase (GK) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P32189

Core Product Specifications and Parameters

Biological Background: Glycerol kinase (GK) Function and Localization

• Glycerol kinase (GK), also known as ATP:glycerol 3-phosphotransferase, is a key enzyme in glycerol metabolism that catalyzes the phosphorylation of glycerol to sn-glycerol 3-phosphate.
• Sn-glycerol 3-phosphate serves as a critical branch-point intermediate in multiple metabolic pathways, including glycerolipid and triglyceride synthesis, glycogenesis, glycolysis, and gluconeogenesis.
• The human GK gene undergoes alternative splicing, resulting in multiple protein isoforms with tissue-specific expression patterns.
• GK is widely expressed in both fetal and adult tissues; notably, a single isoform accounts for all GK expression in adult liver and kidney.
• Subcellularly, GK localizes to the mitochondrial outer membrane, nucleus, and cytosol, reflecting its dynamic role in energy and lipid homeostasis.
• Consistent with an enzymatic function, GK binds ATP and requires divalent metal ions (e.g., Zn²⁺) for activity; it is also associated with a transmembrane domain and disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 422–559, a region proximal to the C-terminus of human GK. Consider verifying signal specificity in knockout or knockdown models if available.
• For Western blot (WB), a primary band around 61 kDa is expected. Using a positive control lysate from human, mouse, or rat tissues with known GK expression is recommended.
• In immunohistochemistry (IHC-P), optimal results may require optimization of antigen retrieval conditions and antibody dilution; a tissue known to express GK (e.g., liver or kidney) should serve as a positive control.
• For ELISA, partnering with a validated capture or detection antibody may be necessary depending on the assay format. Cross-reactivity with mouse and rat orthologs allows flexible species selection for validation experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For glycerol kinase (GK) and related metabolic targets, CamelBio can streamline sourcing of rare-target raw materials and offer technical services spanning from concept to clinical application. Contact us to explore how we can accelerate your assay development and secure a stable supply chain for your metabolic disease research needs.