Anti-Glycerol Kinase (GK) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P32189

Core Product Specifications and Parameters

Biological Background: Glycerol kinase Function and Localization

• Glycerol kinase (GK), encoded by the GK gene (GeneID: 2710), is an ATP:glycerol 3-phosphotransferase that belongs to the kinase family (UniProt: P32189). The protein is alternatively spliced and is widely expressed in fetal and adult tissues, with the sole isoform expressed in adult liver and kidney.
• Its primary function is to phosphorylate glycerol to sn-glycerol 3-phosphate, a critical step in glycerol metabolism. This intermediate feeds into multiple pathways, including glycerolipid and triglyceride synthesis, glycogenesis, glycolysis, and gluconeogenesis.
• GK localizes to multiple cellular compartments: the mitochondrial outer membrane, nucleus, cytoplasm, and cytosol, reflecting its involvement in both energy metabolism and lipid biosynthesis.
• The enzyme requires ATP and metal ions (zinc) for activity, as indicated by its ATP- and metal-binding properties. It contains nucleotide-binding domains characteristic of kinases.
• Keywords associated with GK include alternative splicing, disease variant, transferase, and transmembrane helix, suggesting post-translational regulation and membrane association.

Experimental Guidance and Technical Tips

• For Western blotting, consider using a denaturing gel and transfer conditions appropriate for a 61 kDa protein. Use positive control lysates from human, mouse, or rat tissues expressing GK, such as liver or kidney.
• In immunohistochemistry (paraffin), antigen retrieval may be necessary; validate with known GK-positive tissues (e.g., liver, kidney).
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with a suitable detection antibody may be required for sandwich ELISA.
• Since the immunogen spans amino acids 450–559 near the C-terminus, the antibody likely recognizes both full-length and potential splice variants containing this epitope. Validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies your IVD raw-material sourcing by offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents. This anti-glycerol kinase rabbit monoclonal antibody supports research into metabolic pathways, lipid disorders, and diagnostic assay development. Whether you need validated antibody pairs, bulk monoclonal antibodies, or custom sourcing for rare targets, CamelBio provides integrated solutions to accelerate your project from concept to clinic.