Anti-GLYAT/GAT Monoclonal Antibody for WB, IHC-P, ELISA - Q6IB77

Core Product Specifications and Parameters

Biological Background: GLYAT/GAT Function and Localization

• GLYAT/GAT encodes glycine N-acyltransferase, a mitochondrial enzyme that conjugates acyl groups to glycine and glutamine, detoxifying xenobiotics and endogenous organic acids.
• The protein preferentially uses benzoyl-CoA over phenylacetyl-CoA as an acyl donor, forming various N-acylglycines.
• It is predominantly expressed in the liver and kidney, with downregulation observed in hepatocellular carcinoma and other liver cancers.
• Subcellular localization is mitochondrial, consistent with its role in detoxification pathways.
• Additional functional features include acetylation, acyltransferase activity, alternative splicing, and detoxification. The protein has a molecular weight of approximately 33924 Da.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using a loading control appropriate for mitochondrial proteins; validate the antibody in liver or kidney lysates.
• For immunohistochemistry (IHC-P), antigen retrieval may be required for formalin-fixed paraffin-embedded tissues; include appropriate positive and negative controls.
• For ELISA, optimize coating antigen and antibody concentration against the recombinant immunogen.
• Cross-reactivity with mouse and rat indicates potential for use in these species, but validation in the specific experimental system is recommended.
• The antibody is supplied as a recombinant rabbit monoclonal, ensuring batch-to-batch consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For metabolic detoxification and liver cancer research, CamelBio offers validated antibody pairs, optimized monoclonal antibodies like this anti-GLYAT/GAT clone, bulk ancillary reagents, and sourcing for rare-target raw materials. Partner with CamelBio to streamline your assay development and procurement needs.