Anti-GLYAT Polyclonal Antibody for WB, ELISA - Q6IB77

Core Product Specifications and Parameters

Biological Background: GLYAT Function and Localization

• GLYAT (Glycine N-acyltransferase) is a mitochondrial enzyme encoded by the GLYAT gene, also referred to as benzoyl-CoA:glycine N-acyltransferase, and belongs to the acyltransferase family.
• It catalyzes the transfer of an acyl group from acyl-CoA to the N-terminus of glycine and, to a lesser extent, glutamine, forming a variety of N-acylglycines. It shows preference for benzoyl-CoA as an acyl donor, thereby playing a central role in the detoxification of xenobiotics such as benzoic acid and salicylic acid, as well as endogenous organic acids like isovaleric acid.
• Predominantly expressed in the liver and kidney, with protein levels significantly down-regulated in hepatocellular carcinoma and other liver cancers, suggesting a potential link to hepatic metabolism and carcinogenesis.
• Localizes to the mitochondrion, consistent with its role in mitochondrial acyl group metabolism and detoxification pathways.
• Multiple isoforms are produced through alternative splicing, as indicated by the "Alternative splicing" keyword, which may modulate tissue-specific or functional variations.
• Post-translational acetylation has been reported, as noted by the "Acetylation" keyword, potentially influencing enzyme activity or stability.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 1-163 of human GLYAT, suggesting recognition of the native protein. Validate the reactivity in the relevant sample system.
• For WB, consider using liver or kidney lysates as positive controls and confirm a band near the predicted molecular weight of ~34 kDa. Cross-reactivity with mouse and rat allows broader utility, but species-specific validation is advised.
• For ELISA, the antibody may be used as a capture or detection reagent; optimal concentrations and pairing should be experimentally determined.
• Standardize assay conditions and include appropriate negative controls to ensure specificity, especially given the alternative splicing variants.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop supplier of IVD raw materials, providing diagnostic manufacturers, laboratories, and research institutes with streamlined access to validated antibody pairs, optimized polyclonal/monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. The GLYAT Rabbit pAb supports research in detoxification pathways, metabolism, and liver cancer biomarker exploration. Partner with CamelBio to efficiently source the critical reagents needed from concept to clinic.