Anti-Glutathione Synthetase (GSS) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P48637

Core Product Specifications and Parameters

Biological Background: Glutathione Synthetase (GSS) Function and Localization

• The GSS gene encodes glutathione synthetase (glutathione synthase), a key enzyme in glutathione biosynthesis.
• It catalyzes the ATP-dependent formation of glutathione from γ-glutamylcysteine and glycine (PubMed:7646467, PubMed:9215686).
  Related references: PMID:7646467 PMID:9215686
• Glutathione is the most abundant intracellular thiol, crucial for antioxidant defense, detoxification, and maintaining redox homeostasis (PubMed:10369661).
  Related references: PMID:10369661
• GSS additionally participates in ophthalmate biosynthesis in hepatocytes (By similarity).
• The enzyme contains ATP- and magnesium-binding sites, characteristic of the ATP-grasp ligase family.
• Post-translational modifications such as acetylation and phosphorylation have been identified on GSS.
• Mutations in GSS are associated with glutathione synthetase deficiency, leading to hereditary hemolytic anemia.
• Alternative splicing of GSS produces multiple isoforms, potentially affecting enzyme function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 350–450 of human GSS, targeting a region that may be well-conserved across species, as indicated by cross-reactivity with mouse and rat.
• For Western blotting, an expected band near 52 kDa should be observed; consider using appropriate loading controls and validating in the relevant sample system.
• In immunofluorescence/ICC applications, optimize fixation and permeabilization protocols to preserve the epitope, and confirm specificity using knockdown or knockout controls where possible.
• For ELISA, pair with a matched detection antibody if used in sandwich format; direct or indirect ELISA can be employed to quantify GSS levels.
• Given the rabbit monoclonal nature, consistent lot-to-lot reproducibility is generally expected, but independent validation in the user's experimental context is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, research labs, and institutes from concept to clinic. We offer validated rabbit monoclonal antibodies like this anti-Glutathione Synthetase (GSS) clone, along with matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets in glutathione metabolism and oxidative stress pathways. Contact us to streamline your raw-material procurement and accelerate assay development.