Anti-Glutaminase (GLS) Rabbit Monoclonal Antibody for WB - O94925

Core Product Specifications and Parameters

Biological Background: Glutaminase (GLS) Function and Localization

• Encoded by the GLS gene (also known as GLS1 or KIAA0838), this enzyme produces multiple isoforms (1-3) through alternative splicing, with isoform 1 being the kidney-type glutaminase.
• Isoform 1 catalyzes the first reaction in the primary pathway for renal catabolism of glutamine, playing a key role in maintaining acid-base homeostasis and regulating the levels of the neurotransmitter glutamate (PubMed:30239721, PubMed:30575854, PubMed:30970188). Related references: PMID:30239721, PMID:30575854, PMID:30970188
• A distinct isoform has been reported to lack catalytic activity, highlighting functional diversity among splice variants.
• Subcellular localization is primarily mitochondrial, with presence in the matrix and also detected in the cytoplasm and cytosol.
• Isoform 1 is highly expressed in brain and kidney, while isoform 3 is abundant in heart and pancreas and also found in certain brain tumors; isoform 2 is expressed in cardiac and skeletal muscle.
• Post-translational modifications include acetylation and phosphorylation; the protein contains ANK repeats and is associated with disease variants linked to epilepsy and cataract.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human GLS, which may affect isoform specificity; consider verifying reactivity with target isoforms in your system.
• For Western blotting, the predicted molecular weight is approximately 73 kDa (UniProt: 73461 Da), but minor variations due to post-translational modifications or isoform differences may occur.
• In immunofluorescence/ICC, expect a mitochondrial staining pattern; validate with appropriate markers in relevant cell types.
• For ELISA or other applications, optimize antibody dilution and confirm performance using lysates from tissues with known expression (e.g., kidney or brain for isoform 1).

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides diagnostic manufacturers and research institutes with streamlined access to validated monoclonal and polyclonal antibodies, antibody pairs, and bulk ancillary reagents. This anti-GLS antibody supports research into renal function, neurotransmitter metabolism, and cancer biology. Whether you need rare-target sourcing or comprehensive supply solutions, CamelBio bridges the gap from concept to clinic.