Anti-GLUT4 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P14672

Core Product Specifications and Parameters

Biological Background: GLUT4 Function and Localization

• GLUT4, encoded by the SLC2A4 gene, is a member of the solute carrier family 2 (facilitated glucose transporter). It is also known as insulin-responsive glucose transporter type 4.
• It functions as an insulin-regulated facilitative glucose transporter, critical for glucose uptake from the blood into muscle and adipose tissue.
• In the basal state, GLUT4 is retained intracellularly in storage compartments; upon insulin stimulation, it translocates to the cell membrane to facilitate glucose influx.
• Subcellular localization dynamically cycles between intracellular perinuclear/endomembrane compartments and the cell surface, dependent on insulin signaling.
• Highly expressed in insulin-sensitive tissues, including skeletal muscle, cardiac muscle, and brown and white adipose tissue.
• Post-translational modifications include phosphorylation, palmitoylation, and ubiquitination; these regulate trafficking and stability. Alterations in GLUT4 function are associated with diabetes mellitus and insulin resistance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 450–509 within the C-terminal region of human GLUT4. This polyclonal antibody may recognize multiple splice variants if expressed.
• For Western blotting, a band of approximately 55 kDa is expected. Validate in human, mouse, or rat lysates.
• In immunofluorescence (IF/ICC, IF-P), monitor translocation from intracellular compartments to the cell membrane upon insulin stimulation in appropriate cell lines or tissue sections.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and detection conditions using skeletal or cardiac muscle or adipose tissue sections.
• ELISA applications may require optimization of coating antigen and detection method; consider paired antibody strategies for sandwich assays.
• As a rabbit polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For glucose metabolism and diabetes research, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-GLUT4 antibody, bulk ancillary reagents, and sourcing of rare targets to support your assay development and clinical translation.