Anti-GLUT4 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P14672

Core Product Specifications and Parameters

Biological Background: GLUT4 Function and Localization

• GLUT4, encoded by the SLC2A4 gene, is a member of the solute carrier family 2 (facilitated glucose transporter) and is also known as glucose transporter type 4.
• It functions as an insulin-regulated facilitative glucose transporter, critical for removing glucose from the bloodstream.
• In the absence of insulin, GLUT4 is efficiently retained in intracellular storage compartments within muscle and fat cells.
• Upon insulin stimulation, GLUT4 translocates from these compartments to the cell surface, enabling glucose influx.
• Subcellular localization includes the cell membrane, endomembrane system, cytoplasm, and perinuclear region.
• Tissue specificity: predominantly expressed in skeletal and cardiac muscles, as well as brown and white adipose tissue.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from residues 210–310; therefore, the antibody is expected to recognize linear epitopes, suitable for denaturing Western blot protocols.
• For WB, use standard denaturing and reducing conditions; expect a specific band at approximately 55 kDa (theoretical MW ~54.8 kDa) in human and mouse samples.
• For IF/ICC and IHC-P, consider testing both untreated and insulin-stimulated cells/tissues to observe GLUT4 translocation.
• For ELISA, the peptide immunogen may be used as a positive control to validate antibody binding; assay conditions should be optimized.
• This antibody is indicated to cross-react with human and mouse; we recommend confirming reactivity in your specific tissue or cell line.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, supporting glucose metabolism research with high-quality antibodies like this anti-GLUT4 polyclonal antibody. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic assay development. We also offer custom sourcing for rare targets, helping you efficiently advance projects from concept to clinic.