Anti-GLUT2/SLC2A2 Rabbit Monoclonal Antibody for WB and ELISA - P11168

Core Product Specifications and Parameters

Biological Background: GLUT2/SLC2A2 Function and Localization

• GLUT2/SLC2A2, encoded by the SLC2A2 gene, is a member of the facilitative glucose transporter (GLUT) family and is also known as glucose transporter type 2, liver (GLUT-2).
• It functions as a hexose transporter facilitating the uptake of glucose, fructose, and galactose. Related references: PMID:16186102 PMID:23396969 PMID:28083649
• In hepatocytes, it mediates bidirectional glucose flux, and in pancreatic beta cells, it is essential for glucose uptake and sensing. Related references: PMID:8027028
• It contributes to transcellular glucose transport in the small intestine and kidney, potentially working alongside Na+/glucose cotransporters. Related references: PMID:3399500
• Beyond sugars, GLUT2/SLC2A2 can transport dehydroascorbate (vitamin C) and urate. Related references: PMID:23396969 PMID:40209957
• The protein localizes to the plasma membrane and is primarily expressed in the liver, pancreatic beta cells, small intestine, and kidney.
• It undergoes alternative splicing and post-translational modifications including glycosylation and phosphorylation, as noted in UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (amino acids 1-100) of human GLUT2/SLC2A2, a domain conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot (WB), consider using whole-cell lysates from liver or pancreatic beta cell lines; the expected band size is approximately 57 kDa. Validate detection under reducing and denaturing conditions.
• For ELISA, this antibody may be employed as a capture or detection reagent. Optimization of coating concentrations and detection conditions is recommended for your specific assay format.
• As a multi-pass membrane protein, GLUT2/SLC2A2 may require careful sample preparation to maintain solubility. Standard SDS-PAGE protocols are generally suitable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research organizations with a streamlined supply chain for IVD raw materials, supporting the development of assays targeting glucose metabolism and transporter biology. We offer validated monoclonal antibodies like this anti-GLUT2 clone, along with matched antibody pairs, recombinant proteins, and bulk ancillary reagents to accelerate your project. With technical consulting available from concept to clinic, CamelBio is your partner for rare target sourcing and end-to-end raw material solutions.