Anti-GLUT1/SLC2A1 Rabbit Polyclonal Antibody for WB and ELISA - P11166

Core Product Specifications and Parameters

Biological Background: GLUT1/SLC2A1 Function and Localization

• GLUT1 (SLC2A1) is a member of the solute carrier family 2 facilitated glucose transporters, also known as GLUT-1, erythrocyte/brain glucose transporter, and HepG2 glucose transporter.
• It functions as a facilitative glucose transporter mediating constitutive basal glucose uptake across the plasma membrane. Related references: PMID:10227690 PMID:10954735 PMID:18245775
• GLUT1 exhibits broad substrate specificity, transporting aldoses including pentoses and hexoses. Related references: PMID:18245775 PMID:19449892
• As the primary glucose transporter at the blood-brain barrier, GLUT1 ensures energy-independent glucose transport into the brain. Related references: PMID:10227690
• In retinal photoreceptors, GLUT1 associates with BSG and NXNL1 to promote cone survival by enhancing glucose uptake (By similarity).
• GLUT1 is essential for mesendoderm differentiation (By similarity).
• Subcellularly, GLUT1 localizes to the cell membrane, melanosomes, and photoreceptor inner segments.
• It is detected in erythrocytes and variably expressed in many human tissues, with post-translational modifications including glycosylation, acetylation, and phosphorylation; mutations are linked to GLUT1 deficiency syndrome, dystonia, epilepsy, and hemolytic anemia.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal cytoplasmic domain (aa 1-400) of human GLUT1; consider epitope accessibility in native versus denaturing conditions when designing experiments.
• For western blotting, the predicted molecular weight of GLUT1 is approximately 54 kDa; validate band specificity using appropriate positive and negative controls.
• In ELISA applications, optimize coating conditions and antibody dilutions; the antibody may detect both glycosylated and unglycosylated forms of GLUT1.
• Due to high sequence conservation, check cross-reactivity if considering use with non-human samples.

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CamelBio offers comprehensive sourcing solutions for IVD raw materials, including validated polyclonal antibodies like this anti-GLUT1/SLC2A1 antibody, essential for research on glucose transport, brain metabolism, and related disorders. Our portfolio spans optimized antibody pairs, recombinant proteins, and bulk ancillary reagents, supporting your assay development and manufacturing needs from concept to clinic.