Anti-GluR4/GluA4/GRIA4 Rabbit Polyclonal Antibody for WB and ELISA - P48058

Core Product Specifications and Parameters

Biological Background: GluR4/GluA4/GRIA4 Function and Localization

• Glutamate receptor 4 (GluR4), also known as AMPA-selective glutamate receptor 4 or GluR-D, is encoded by the GRIA4 gene and belongs to the ionotropic glutamate receptor family.
• Functions as a ligand-gated cation channel activated by L-glutamate and other glutamatergic agonists such as AMPA, quisqualic acid, and kainic acid, playing a critical role in fast excitatory synaptic transmission.
• Upon L-glutamate binding, the receptor undergoes a conformational change, opening the channel to allow influx of monovalent and divalent cations (e.g., Na⁺, Ca²⁺), converting chemical signals into electrical impulses.
• The receptor desensitizes rapidly after activation, entering a transient inactive state; however, in the presence of CACNG8, it exhibits resensitization characterized by delayed accumulation of current flux (PubMed:21172611). Related references: PMID:21172611
• Localized at the cell membrane, specifically the postsynaptic membrane, and also found on dendrites.
• Subject to alternative splicing, generating multiple isoforms, and undergoes post-translational modifications including phosphorylation and palmitoylation.
• Associated with disease variants linked to intellectual disability (UniProt keyword).

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (aa 254-433) of human GluR4. The antibody is expected to recognize multiple isoforms; validation for specific isoforms may be necessary.
• For Western blotting, a band around 101 kDa is anticipated. Consider using appropriate positive controls from human, mouse, or rat brain lysates, given the reported cross-reactivity.
• In ELISA applications, optimization of coating and detection conditions is recommended; the antibody can be used as a capture or detection reagent following standard protocols.
• Due to potential post-translational modifications, band shifts or multiple bands may be observed; include phosphatase inhibitors if needed.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted one-stop IVD raw material partner, CamelBio supports diagnostic manufacturers and research labs with high-quality antibodies like this anti-GluR4 polyclonal antibody, facilitating studies on glutamatergic signaling and synaptic function. We offer comprehensive sourcing services, including validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents, ensuring reliable supply for assay development and production. Our expertise bridges the gap from concept to clinic, providing tailored technical support and rare target raw-material procurement to accelerate your IVD projects.