Anti-GluR4/GluA4/GRIA4 Rabbit Monoclonal Antibody for WB and ELISA - P48058

Core Product Specifications and Parameters

Biological Background: Glutamate Receptor 4 Function and Localization

• Glutamate receptor 4 (GluR4, GRIA4) is a subunit of AMPA-selective ionotropic glutamate receptors, which function as ligand-gated cation channels activated by L-glutamate and synthetic agonists such as AMPA.
• It mediates fast excitatory synaptic transmission in the central nervous system by converting chemical signals into electrical impulses upon cation influx.
• The receptor undergoes rapid desensitization after activation; however, co-assembly with the auxiliary subunit CACNG8 induces resensitization, leading to a delayed accumulation of current during sustained agonist application. Related references: PMID:21172611
• GRIA4 belongs to a family of four genes (GRIA1–4) encoding AMPA receptor subunits, which combine to form tetrameric channels with distinct functional properties.
• The protein is localized at postsynaptic membranes of dendrites, where it participates in synaptic plasticity and neuronal communication.
• Post-translational modifications, including glycosylation, palmitoylation, phosphorylation, and disulfide bond formation, regulate receptor trafficking, stability, and function.
• Mutations in GRIA4 are associated with neurodevelopmental phenotypes, including autosomal dominant intellectual developmental disorder (NEDSGA).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 800–902 in the intracellular C-terminal domain; consider that this region may be subject to alternative splicing or post-translational modifications, which could influence antibody binding.
• For Western blot, the predicted molecular weight of GluR4 is ~101 kDa. Validate detection in relevant tissue or cell lysates, and consider the use of appropriate loading controls.
• The antibody is cross-reactive with mouse and rat GluR4, supporting its use in comparative neurobiology studies across species.
• ELISA protocols should be optimized by titration of both coating antigen and antibody under standard conditions; ensure the synthetic peptide or recombinant protein used matches the immunogen sequence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio consolidates sourcing of diagnostic-grade raw materials, offering validated antibody pairs, monoclonal antibodies, and ancillary reagents for neuroscience and ion channel research. This anti-GluR4 antibody supports studies on excitatory synaptic transmission and receptor function, with flexible supply options and technical support for assay development. Connect with us to leverage integrated procurement and accelerate your workflow from discovery to clinical application.