Anti-GluR2/GRIA2 Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P42262

Core Product Specifications and Parameters

Biological Background: GluR2/GRIA2 Function and Localization

• GluR2/GRIA2 is a subunit of AMPA-type ionotropic glutamate receptors, which are ligand-gated cation channels activated by L-glutamate and agonists like AMPA, quisqualate, and kainate. Related references: PMID:20614889 PMID:31300657 PMID:8003671
• It mediates fast excitatory synaptic transmission in the central nervous system by allowing Na+ and Ca2+ influx upon glutamate binding. Related references: PMID:14687553
• The receptor undergoes rapid desensitization after activation and can resensitize in the presence of auxiliary subunits such as CACNG4, CACNG7, or CACNG8.
• GluR2 is a component of the postsynaptic density membrane, localizing to excitatory synapses where it participates in synaptic plasticity and signal transduction.
• Post-translational modifications include glycosylation, palmitoylation, phosphorylation, RNA editing, and ubiquitination, which regulate receptor trafficking and function.
• It interacts with proteins such as NSG1, GRIP1, and STX12 to control endosomal sorting and membrane targeting of AMPA receptors.
• Mutations in GRIA2 are associated with neurological disorders including autism spectrum disorder, epilepsy, and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to amino acids 150–250 within the extracellular N‑terminal domain of human GluR2/GRIA2, a region predicted to be solvent‑accessible in the native receptor.
• In Western blotting, a predominant band near 99 kDa is expected; alternative splicing, post‑translational modifications, or protein complexes may produce additional bands. Optimize lysate preparation and antibody dilution for your specific sample type.
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF‑P), standard antigen retrieval methods and permeabilization steps may be required; consider validating with known positive and negative control tissues.
• This antibody cross‑reacts with mouse and rat GluR2/GRIA2, making it suitable for translational studies across these species; however, we recommend verifying reactivity in your specific model system.
• ELISA applications may benefit from using the antibody as a capture or detection reagent; titrate carefully and include appropriate controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one‑stop sourcing bridge for IVD raw materials, offering validated monoclonal antibodies like this anti‑GluR2/GRIA2 antibody to support neuroscience and synaptic biology research. Beyond individual reagents, we provide optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare‑target raw materials, empowering diagnostic manufacturers and research laboratories from concept to clinic. Partner with CamelBio to streamline your supply chain and accelerate development.