Anti-GluR1/GRIA1 Rabbit Monoclonal Antibody for WB, ELISA - P42261

Core Product Specifications and Parameters

Biological Background: GluR1/GRIA1 Function and Localization

• Glutamate receptor 1 (GluR1/GRIA1) is an AMPA-selective ionotropic glutamate receptor, also known as GluR-A, GluR-K1, or AMPA-selective glutamate receptor 1, encoded by the GRIA1 gene. It belongs to the glutamate-gated ion channel family and mediates fast excitatory neurotransmission in the central nervous system.
• Functions as a ligand-gated ion channel activated by L-glutamate and the synthetic agonist AMPA, as well as quisqualic acid and kainic acid. Upon agonist binding, the channel opens, allowing influx of monovalent (Na⁺, K⁺, Li⁺) and divalent (Ca²⁺, Mg²⁺, Sr²⁺) cations, thereby depolarizing the postsynaptic membrane (PubMed:1311100, PubMed:20805473). Related references: PMID:1311100, PMID:20805473
• In complex with auxiliary subunits CACNG2, CACNG4, CACNG7, or CACNG8, GluR1 undergoes resensitization – a delayed accumulation of current upon sustained agonist application. This process is inhibited by CNIH2 through interaction with CACNG8 (PubMed:21172611). Related references: PMID:21172611
• Calcium permeability is dependent on subunit composition; heteromeric channels that include an edited GRIA2 subunit are calcium-impermeable, whereas GluR1 homomers or complexes lacking edited GRIA2 allow calcium entry.
• Subcellular localization includes the postsynaptic cell membrane, postsynaptic density, dendritic spines, and dendrites; it is also detected in early and recycling endosomes, the endoplasmic reticulum, and presynaptic compartments, reflecting dynamic trafficking.
• Key post-translational modifications include N-glycosylation, phosphorylation, palmitoylation, and disulfide bond formation, which regulate channel function, trafficking, and synaptic plasticity.
• Widely expressed in the brain, with no significant peripheral tissue distribution.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 800–906 within the intracellular C‑terminal domain of human GluR1/GRIA1. This region is conserved across mouse and rat, supporting the observed cross‑reactivity.
• For Western blot, expect a band at approximately 102 kDa. Due to glycosylation and other modifications, slight shifts may occur; validate using appropriate positive and negative controls.
• For ELISA, the antibody may be used as a capture or detection reagent; consider pairing with a second antibody recognizing a distinct epitope or using recombinant GluR1 protein as a standard.
• Since the antibody recognizes a linear epitope, denaturing and reducing conditions in WB should be effective. However, for native detection (e.g., immunohistochemistry), test under non‑denaturing conditions and include appropriate tissue controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for IVD raw‑material procurement, supporting neuroscience and receptor biology programs with high‑quality antibodies and ancillary reagents. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and custom sourcing for rare targets, empowering diagnostic manufacturers and researchers from concept to clinic. Whether you need bulk GluR1 reagents or tailored technical consultation, CamelBio streamlines your supply chain.