Anti-GluR1/GRIA1 Polyclonal Antibody for WB, IHC-P, ELISA - P42261

Core Product Specifications and Parameters

Biological Background: Glutamate receptor 1 Function and Localization

• GluR1/GRIA1 is an ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and AMPA receptor agonists. Upon agonist binding, the channel opens, allowing influx of monovalent and divalent cations such as sodium and calcium to mediate fast excitatory neurotransmission. Related references: PMID:1311100, PMID:20805473, PMID:21172611
• In the presence of auxiliary subunits (CNIH2, CACNG2/4/7/8), AMPA receptors exhibit resensitization—a delayed accumulation of current upon continued agonist application—which is modulated by transmembrane AMPA receptor regulatory proteins (TARPs). Related references: PMID:21172611
• Calcium permeability depends on subunit composition; heteromeric channels containing edited GRIA2 are calcium-impermeable, whereas GluR1 homomers or combinations with unedited subunits allow calcium entry.
• The receptor localizes to multiple subcellular compartments including the cell membrane, endoplasmic reticulum, postsynaptic density, dendritic spines, early endosomes, recycling endosomes, and presynaptic terminals, highlighting its dynamic trafficking and role in synaptic plasticity.
• GluR1 is widely expressed in the brain, and mutations in the GRIA1 gene are associated with neurological disorders such as intellectual disability (MRD67/MRT76).
• Extensive post-translational regulation occurs via phosphorylation, palmitoylation, and glycosylation, which modulate receptor trafficking, activity, and stability.
• Encoded by the GRIA1 gene on human chromosome 5, the transcript undergoes alternative splicing to generate multiple isoforms.

Experimental Guidance and Technical Tips

• For western blotting (WB), the predicted molecular weight is ~102 kDa. Use reducing agents and boiling to ensure proper denaturation and detection of the monomeric band.
• Validate the antibody in the relevant sample system: brain or neuronal tissue lysates from human, mouse, or rat are recommended as positive controls.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and permeabilization conditions, as the target is a transmembrane protein with multiple membrane-spanning domains.
• In ELISA applications, consider pairing this antibody with a detection antibody raised against a different epitope to build a sandwich assay.
• The immunogen (amino acids 330-470) corresponds to a conserved extracellular or intracellular region; verify epitope accessibility in your specific assay format.

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CamelBio is your integrated partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target procurement to support neuroscience and synaptic research. With a comprehensive portfolio and end-to-end technical support, we help diagnostic developers and researchers accelerate their projects from concept to clinic. Explore our GluR1/GRIA1 antibody and related neuroscience reagents to streamline your assay development.