Anti-GLUD2 Rabbit Polyclonal Antibody - P49448

Core Product Specifications and Parameters

Biological Background: GLUD2 Function and Localization

• GLUD2 (glutamate dehydrogenase 2) is a mitochondrial enzyme that recycles the chief excitatory neurotransmitter, glutamate, during neurotransmission.
• The protein is encoded by the GLUD2 gene (also known as GDH2, GLUDP1) and shares high homology with its housekeeping counterpart GLUD1.
• GLUD2 localizes to the mitochondrial matrix, consistent with its role in central metabolism and neurotransmitter handling.
• Tissue expression is highest in retina and testis, with lower levels detected in brain.
• UniProt keywords include oxidoreductase, NADP, and ADP-ribosylation, indicating involvement in redox reactions and potential post-translational regulation.
• The protein contains a transit peptide (cleaved during mitochondrial import) and has a mature molecular weight of approximately 61 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 319–558), so the antibody may recognize full-length GLUD2 and could be suitable for detecting the mature protein in mitochondrial lysates.
• For Western blot (WB), consider using reducing conditions and a loading control; the predicted band is around 61 kDa. Validate specificity in tissues with known expression (e.g., human retina, testis).
• For immunohistochemistry (IHC-P), test antigen retrieval protocols and antibody dilutions on positive control tissues such as retina or testis; a mitochondrial staining pattern is expected.
• In immunofluorescence (IF/ICC), permeabilization may be required to access the mitochondrial matrix; co-stain with a mitochondrial marker to confirm localization.
• For ELISA, the antibody can be used as a capture or detection reagent after appropriate optimization; cross-reactivity with GLUD1 should be checked if GLUD2 specificity is critical.
• Given cross-reactivity with mouse and rat, the antibody is useful for studies in rodent models of glutamate metabolism.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers, laboratories, and research institutes with convenient, one-stop access to IVD raw materials and technical expertise. The anti-GLUD2 polyclonal antibody supports research into neurotransmitter metabolism and mitochondrial dysfunction, aligning with our portfolio of validated antibody pairs, optimized mono/polyclonal reagents, bulk ancillary supplies, and rare target sourcing. Our team is ready to assist with customized procurement to accelerate your diagnostic or R&D workflows.