Anti-GLUD1/2 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P00367/P49448

Core Product Specifications and Parameters

Biological Background: GLUD1/2 Function and Localization

• GLUD1 (glutamate dehydrogenase 1) is a mitochondrial matrix enzyme that catalyzes the reversible oxidative deamination of L-glutamate to alpha-ketoglutarate and ammonia, using NAD(P)+ as a cofactor.
• It plays a key role in glutamine anaplerosis, producing alpha-ketoglutarate to replenish tricarboxylic acid cycle intermediates. Related references: PMID:11032875 PMID:11254391 PMID:16023112
• The enzyme is involved in insulin homeostasis, regulating glucose-stimulated insulin secretion in pancreatic beta cells. Related references: PMID:11297618 PMID:9571255
• It may modulate the turnover of the excitatory neurotransmitter glutamate, potentially influencing learning and memory processes.
• GLUD1 localizes primarily to the mitochondrial matrix and has also been detected at the endoplasmic reticulum.
• The protein undergoes post-translational modifications including acetylation, ADP-ribosylation, and phosphorylation, which regulate its activity.
• This antibody targets both GLUD1 and its isoform GLUD2 (also known as GDH2), due to the high sequence homology between the two proteins; GLUD2 is predominantly expressed in neural and testicular tissues.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a recombinant protein covering amino acids 319–558 of human GLUD1/2, a region conserved across both isoforms. It is expected to detect both endogenous GLUD1 and GLUD2.
• For Western blotting, a band at approximately 61 kDa should be observed in lysates from human, mouse, and rat samples. Use appropriate positive controls such as liver or brain tissue extracts.
• In immunostaining (IF/ICC, IHC-P), mitochondrial localization is anticipated. A punctate perinuclear pattern may be visible; counterstaining with a mitochondrial marker can help confirm specificity.
• The antibody is applicable to WB, IF/ICC, IF-P, IHC-P, and ELISA. Optimal working concentrations and conditions should be determined empirically for each assay and sample type.
• Due to the high similarity between GLUD1 and GLUD2, cross-reactivity with both targets is expected; this should be considered when interpreting results in tissues where both isoforms are expressed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined one-stop solution for IVD raw material sourcing. Beyond this anti-GLUD1/2 antibody, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized procurement of rare target raw materials. For metabolic and neurological research involving glutamate dehydrogenase, CamelBio supports your workflow from concept to clinic, ensuring reliable supply so you can accelerate innovation in diagnostic kit development.