Anti-GLUD1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P00367

Core Product Specifications and Parameters

Biological Background: GLUD1 Function and Localization

• GLUD1 encodes mitochondrial glutamate dehydrogenase 1, a key enzyme that reversibly converts L-glutamate to alpha-ketoglutarate, bridging amino acid and carbohydrate metabolism.
  Related references: PMID:11032875, PMID:11254391, PMID:16023112
• It plays a critical role in glutamine anaplerosis by replenishing TCA cycle intermediates, essential for rapidly proliferating cells.
  Related references: PMID:11032875, PMID:11254391, PMID:16023112
• GLUD1 participates in insulin homeostasis, regulating glucose-stimulated insulin secretion from pancreatic β-cells.
  Related references: PMID:11297618, PMID:9571255
• The protein localizes primarily to the mitochondrial matrix, with minor detection in the endoplasmic reticulum.
• Multiple post-translational modifications, including acetylation, ADP-ribosylation, phosphorylation, and hydroxylation, fine-tune its enzymatic activity.
• Mutations in GLUD1 are associated with congenital hyperinsulinism-hyperammonemia syndrome, underscoring its clinical significance.
• It is a member of the NADP-dependent oxidoreductase family and is expressed across a broad range of tissues.

Experimental Guidance and Technical Tips

• The immunogen (aa 459–558) lies within a conserved C‑terminal region; verify antibody specificity via peptide competition or siRNA knockdown.
• For WB, the predicted molecular weight is ~61 kDa; use whole-cell lysates from human, mouse, or rat tissues as positive controls.
• In IHC‑P, optimize antigen retrieval and validate staining in GLUD1‑rich tissues such as liver and pancreatic islets.
• For IF/ICC, choose fixation and permeabilization protocols that preserve mitochondrial morphology.
• ELISA applications may require titration of coating antigen and detection conditions for optimal signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw‑material solutions, including validated monoclonal antibodies like this anti‑GLUD1 clone for metabolic and neuroscience research. Beyond antibodies, we supply matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets — streamlining your assay development pipeline.