Anti-Glucosylceramidase beta (GBA) Monoclonal Antibody for WB, IHC-P, ELISA - P04062

Core Product Specifications and Parameters

Biological Background: Glucosylceramidase beta (GBA) Function and Localization

• Catalyzes lysosomal hydrolysis of glucosylceramides (GlcCers) into free ceramide and glucose, a critical step in sphingolipid catabolism. Related references: PMID:15916907, PMID:24211208, PMID:32144204
• Essential for the degradation of complex lipids and turnover of cellular membranes. Related references: PMID:27378698
• Participates in the PKC-activated salvage pathway of ceramide formation by providing ceramide from GlcCer breakdown. Related references: PMID:19279011
• Catalyzes transglucosylation of cholesterol, using GlcCer as glucose donor, to form cholesteryl glucoside, and can also hydrolyze cholesteryl glucoside back to cholesterol and glucose. Related references: PMID:24211208, PMID:26724485, PMID:32144204
• Exhibits alternative glycosidase/transferase activities toward galactosylceramide (GalCer) and xylosylceramide (XylCer) substrates, although with lower activity than with GlcCer. Related references: PMID:32144204, PMID:33361282, PMID:39395789
• Localized to the lysosome membrane; mutations in GBA lead to Gaucher disease and are associated with Parkinsonism.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 437–536, a region likely to be accessible in both native and denatured GBA.
• For Western blot, expect a band near 60 kDa; validate specificity using relevant human or rat lysates.
• For IHC-P, optimize antigen retrieval to unmask the epitope and confirm staining in positive control tissues.
• For ELISA, consider pairing with a detection antibody and validate using recombinant GBA or known positive samples.
• Cross-reactivity with rat enables interspecies studies; validate expression levels in the target rat tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents and rare target sourcing. This anti-GBA antibody supports research into lysosomal storage disorders, lipid metabolism, and neurodegenerative diseases. Reach out to leverage our comprehensive portfolio and sourcing expertise for your next project.