Anti-Glucose-6-Phosphate Isomerase (GPI) Monoclonal Antibody [KD Validated] for WB, IF/ICC, ELISA - P06744

Core Product Specifications and Parameters

Biological Background: Glucose-6-Phosphate Isomerase Function and Localization

• Glucose-6-phosphate isomerase (GPI) is also known as autocrine motility factor (AMF), neuroleukin (NLK), phosphoglucose isomerase (PGI), phosphohexose isomerase (PHI), and sperm antigen 36 (SA-36).
• The enzyme catalyzes the conversion of alpha-D-glucose-6-phosphate to beta-D-fructose-6-phosphate, the second step in glycolysis, and the reverse reaction in gluconeogenesis, within the cytoplasm. Related references: PMID:28803808
• GPI also exhibits C2-epimerase activity, interconverting D-glucose-6-phosphate and D-mannose-6-phosphate, and anomerase activity for multiple hexose-6-phosphates.
• Besides its metabolic role, extracellular GPI functions as a cytokine: as autocrine motility factor (AMF), a secreted angiogenic factor enhancing endothelial cell motility. Related references: PMID:11437381
• It also acts as neuroleukin, a neurotrophic factor supporting spinal and sensory neuron survival, and is released by lectin-stimulated T-cells to induce immunoglobulin secretion. Related references: PMID:11004567 PMID:3352745
• Subcellularly, GPI is found in the cytoplasm and is secreted.
• Post-translational modifications include acetylation, hydroxylation, phosphorylation, and ubiquitin conjugation; alternative splicing produces multiple isoforms.
• Mutations in GPI are associated with hereditary hemolytic anemia.

Experimental Guidance and Technical Tips

• The antibody was generated using a recombinant immunogen encompassing amino acids 1-280 of human GPI (NP_000166.2), which may target conserved epitopes; cross-reactivity with mouse and rat is confirmed.
• For Western blotting (WB), a band around 63 kDa is expected; consider using reducing conditions and appropriate positive controls such as human, mouse, or rat cell lysates.
• In immunofluorescence/immunocytochemistry (IF/ICC), note that GPI is cytoplasmic and secreted; fixation and permeabilization may be required for intracellular detection, and consider co-staining with organelle markers.
• For ELISA, the antibody may be used as capture or detection reagent; validate with recombinant GPI protein or biological samples to establish linearity and sensitivity.
• As a monoclonal antibody, batch-to-batch consistency is expected, but always optimize working dilutions for your specific assay and sample type.
• The antibody is labeled as KD validated; consider incorporating appropriate knockdown controls in your experiments to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing access to a wide range of research-grade antibodies, antigens, and ancillary reagents. Whether you need validated antibody pairs for diagnostic assay development, bulk monoclonals for manufacturing, or specialized reagents for metabolic and cytokine research, CamelBio streamlines procurement from concept to clinic. Our product portfolio includes key targets like GPI/AMF, enabling critical studies in glycolysis, cell motility, and neuroscience.