Anti-Glucocorticoid Receptor Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P04150

Core Product Specifications and Parameters

Biological Background: Glucocorticoid Receptor Function and Localization

• Glucocorticoid receptor (GR), encoded by the NR3C1 gene, is a member of the nuclear receptor subfamily 3 group C. Multiple isoforms arise from alternative splicing and initiation, with isoform Alpha being the canonical full-length receptor.
• Functions as a receptor for glucocorticoids, acting as a ligand-activated transcription factor that binds glucocorticoid response elements (GREs) to regulate gene expression, and modulating other transcription factors to control inflammation, proliferation, and differentiation. Related references: PMID:27120390, PMID:37478846, PMID:28139699
• Involved in chromatin remodeling and post-transcriptional regulation by binding the 5' UTR of target mRNAs and recruiting the RNA decay machinery. Related references: PMID:9590696, PMID:25775514
• Mediates glucocorticoid-induced apoptosis and contributes to accurate chromosome segregation during mitosis, with potential tumor suppressor functions. Related references: PMID:23303127, PMID:25847991
• Predominantly cytoplasmic in the absence of ligand; translocates to the nucleus upon activation. Also detected in mitochondria, the mitotic spindle, centrosome, and nucleoplasm.
• Subject to extensive post-translational modifications including phosphorylation, acetylation, methylation, SUMOylation, and ubiquitination, which fine-tune its transcriptional activity and stability.
• Widely expressed across human tissues, with high levels in bone, lung, liver, kidney, and immune-related organs; cardiac expression includes atria, ventricles, and conduction system.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 1–280 amino acids of human GR (isoform Alpha-2, NP_001191194.1), covering the AF-1 transactivation domain. Consider verifying the antibody's performance in your specific sample type and experimental conditions.
• For Western blot, a band around 86 kDa is expected; using nuclear extracts or glucocorticoid-stimulated cells may enhance detection.
• For IHC-P and IF/ICC, optimize fixation and antigen retrieval conditions. Validate antibody specificity in relevant tissue/cell models.
• The antibody's reported cross-reactivity with mouse supports translational studies, but validation in target mouse tissues or cells is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a one-stop portfolio of IVD raw materials. This anti-Glucocorticoid Receptor polyclonal antibody exemplifies our commitment to providing validated reagents for studying glucocorticoid signaling, apoptosis, and chromatin biology. Beyond antibodies, we supply validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate your assay development and biomarker discovery workflows.