Anti-Glucocorticoid Receptor Monoclonal Antibody for WB, IP, ELISA - P04150

Core Product Specifications and Parameters

Biological Background: Glucocorticoid Receptor Function and Localization

• Glucocorticoid receptor (GR), encoded by the NR3C1 gene, is a member of the nuclear receptor subfamily 3 group C. It functions as a receptor for glucocorticoids and has dual roles as a transcription factor binding to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Related references: PMID:27120390 PMID:37478846
• GR affects inflammatory responses, cellular proliferation, and differentiation, and is involved in chromatin remodeling. Related references: PMID:9590696
• The receptor can also mediate rapid mRNA degradation by binding to 5' UTRs of target mRNAs and recruiting RNA decay machinery in a ligand-dependent manner. Related references: PMID:25775514
• GR has transcriptional activation and repression activities, mediating glucocorticoid-induced apoptosis and promoting accurate chromosome segregation during mitosis; it may also act as a tumor suppressor. Related references: PMID:11435610 PMID:15769988 PMID:15866175 PMID:23303127 PMID:25847991
• Multiple isoforms of GR exist due to alternative initiation and splicing, exhibiting varying transcriptional activities. For example, isoform Alpha has strong transcriptional activity, while isoform Beta acts as a dominant negative inhibitor. Related references: PMID:20484466 PMID:7769088 PMID:8621628 PMID:19248771 PMID:26711253
• Subcellular localization of GR is dynamic: predominantly cytoplasmic in the absence of ligand, it translocates to the nucleus upon activation, and is also found in mitochondria, at the mitotic spindle, and centrosome.
• GR is widely expressed across tissues including bone, stomach, lung, liver, colon, breast, ovary, pancreas, kidney, and heart. In the heart, expression is detected in atria, ventricles, and conduction system. Related references: PMID:25847991 PMID:10902803
• Key post-translational modifications include phosphorylation, acetylation, ubiquitination, and SUMOylation, which regulate GR stability, localization, and transcriptional activity. The protein belongs to the nuclear hormone receptor family and features zinc-finger DNA-binding domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 2-180) of the human glucocorticoid receptor. This region is shared by several isoforms, so the antibody may detect multiple isoforms if they retain this sequence.
• For WB, a band around 86kDa is expected for the full-length receptor; consider using appropriate controls and molecular weight markers.
• In IP, optimize antibody concentration and buffer conditions for efficient pull-down of GR complexes.
• For ELISA, validate the antibody with recombinant protein or cell lysates expressing GR to ensure specificity and sensitivity.
• Cross-reactivity with mouse and rat suggests conserved epitopes; confirm performance in your species of interest by testing in relevant sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For studies on glucocorticoid receptor signaling, apoptosis, and chromatin remodeling, our sourcing platform delivers validated antibody pairs, optimized monoclonal antibodies like this anti-GR rabbit mAb, bulk ancillary reagents, and custom raw-material procurement for rare targets. Streamline your diagnostic assay development with CamelBio's integrated supply solutions.