Anti-Glucagon Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P01275

Core Product Specifications and Parameters

Biological Background: Pro-glucagon Function and Localization

• Pro-glucagon (GCG) is a precursor polypeptide that undergoes tissue-specific proteolytic cleavage to generate several peptide hormones, including glucagon, glucagon-like peptide-1 (GLP-1), GLP-2, glicentin, and oxyntomodulin.
• Glucagon plays a key role in glucose metabolism by opposing insulin action: it raises blood glucose levels through stimulation of hepatic gluconeogenesis and glycogenolysis. Related references: PMID:32193322
• GLP-1 is a potent incretin hormone that stimulates glucose-dependent insulin secretion from pancreatic beta cells, suppresses appetite, and delays gastric emptying. Related references: PMID:22037645, PMID:40446798, PMID:10605628, PMID:14719035, PMID:12554744
• GLP-2 promotes intestinal growth by stimulating crypt cell proliferation and villus height increase, while also enhancing nutrient absorption and reducing enterocyte apoptosis.
• Pro-glucagon is highly expressed in pancreatic islet A cells, intestinal enteroendocrine L cells, and specific brain neurons, reflecting its diverse endocrine and neuromodulatory functions. Related references: PMID:22037645
• The mature peptides are generated via prohormone convertase cleavage at paired basic residues and are post‑translationally modified by C‑terminal amidation, with phosphorylation also detected.

Experimental Guidance and Technical Tips

• The immunogen was designed using a synthetic peptide spanning the region encoding glucagon and adjacent sequences (amino acids 21–120 of human pro-glucagon). For WB, a band around 21 kDa is expected; consider a 12% SDS‑PAGE gel for optimal separation.
• For IHC-P and IF-P, antigen retrieval with citrate buffer (pH 6.0) or EDTA (pH 9.0) may enhance staining; validate in relevant positive and negative tissues (pancreas, intestine).
• In ELISA, the rabbit monoclonal format provides high affinity and consistency; consider pairing with a validated detection antibody for sandwich assays.
• Assess cross‑reactivity in mouse and rat samples, as indicated; if unexpected bands appear, perform additional blocking or antibody titration.

CamelBio: Your One-Stop Sourcing Bridge

With this anti-glucagon antibody, researchers gain a robust tool for studying glucose homeostasis, gut hormone biology, and metabolic disorders. CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing—streamlining your diagnostic development from concept to clinic.