Anti-Glucagon Rabbit Monoclonal Antibody for IF-P, IHC-P, ELISA - P01275

Core Product Specifications and Parameters

Biological Background: Glucagon Function and Localization

• Pro-glucagon (GCG) is a precursor hormone that is proteolytically cleaved to produce multiple peptide hormones, including glucagon, glucagon-like peptide-1 (GLP-1), GLP-2, and oxyntomodulin. Glucagon itself is a 29-amino acid pancreatic hormone critical for glucose homeostasis.
• Glucagon plays a central role in glucose metabolism by stimulating hepatic gluconeogenesis and glycogenolysis, thereby raising blood glucose levels. It acts as a counterregulatory hormone to insulin, and its dysregulation is implicated in diabetes. Related references: PMID:32193322
• GLP-1 is a potent incretin that promotes glucose-dependent insulin secretion from pancreatic beta cells, suppresses glucagon release, delays gastric emptying, and promotes satiety, thus contributing to glucose control and body weight regulation (PubMed:22037645, PubMed:40446798). Additional roles include neuroendocrine modulation and stimulation of beta cell proliferation (PubMed:10605628, PubMed:14719035, PubMed:12554744). Related references: PMID:22037645, PMID:40446798, PMID:10605628, PMID:14719035, PMID:12554744
• GLP-2 is an intestinotrophic hormone that promotes intestinal growth, enhances nutrient absorption, and maintains gut barrier function. It stimulates crypt cell proliferation and inhibits enterocyte apoptosis.
• The glucagon gene is expressed predominantly in pancreatic A cells of the islets of Langerhans, and in enteroendocrine L cells scattered throughout the gastrointestinal tract. GLP-1 and GLP-2 are also found in selected neurons in the brain.
• Post-translational processing of pro-glucagon involves tissue-specific cleavage by prohormone convertases, as well as C-terminal amidation, which is essential for full biological activity.
• This rabbit monoclonal antibody, raised against a peptide corresponding to the N-terminal region of human pro-glucagon (amino acids 1-100), detects the ~21 kDa precursor protein, and is expected to recognize glucagon and related processed peptides in relevant tissue contexts.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal sequence of human pro-glucagon; consider verifying reactivity with both the full-length precursor and mature glucagon/GLP-1 peptides in your assay.
• For immunohistochemistry (IHC-P), standard paraffin-embedded tissue sections from human pancreas or intestine should be suitable. Antigen retrieval with citrate buffer (pH 6.0) is often beneficial; validation in the target tissue is recommended.
• In immunofluorescence (IF-P), this antibody may label secretory granules in pancreatic α-cells and intestinal L-cells. Co-localization with cell-type-specific markers can aid identification.
• For ELISA, pairing with a detection antibody against a non-overlapping epitope (e.g., C-terminal) may enhance specificity; optimal capture/detection conditions must be determined experimentally.
• Cross-reactivity with mouse and rat has been reported; this enables translational studies in rodent models of diabetes and metabolism. Confirm consistency across species in your laboratory.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers a comprehensive portfolio of IVD raw materials, from validated monoclonal antibodies like this anti-glucagon clone to bulk ancillary reagents and custom sourcing services. For metabolic disorder diagnostics, we offer matched antibody pairs, purified antigens, and technical consultation to streamline assay development. Whether you need a single clone or a complete raw-material package, CamelBio serves as your reliable bridge from target concept to clinical application.