Anti-Glucagon Monoclonal Antibody for IF-P, IHC-P, ELISA - P01275

Core Product Specifications and Parameters

Biological Background: Glucagon Function and Localization

• Glucagon is a key hormone in glucose homeostasis: it raises blood glucose by promoting gluconeogenesis and inhibiting glycolysis, counteracting insulin action, and is critical in diabetic hyperglycemia. Related references: PMID:32193322

• Pro-glucagon gives rise to several bioactive peptides: glucagon, GLP-1, GLP-2, and GRPP, each with distinct metabolic functions.

• GLP-1 stimulates glucose-dependent insulin secretion, inhibits glucagon release, delays gastric emptying, and reduces appetite, playing a role in satiety and peripheral glucose disposal. Related references: PMID:22037645 PMID:40446798 PMID:10605628 PMID:14719035 PMID:12554744

• GLP-2 promotes intestinal growth, increases villus height, enhances nutrient absorption, and reduces enterocyte apoptosis, primarily acting on the gastrointestinal tract.

• Glucagon is secreted from pancreatic α-cells, while GLP-1 and GLP-2 are released from enteroendocrine L cells in the gut and selected neurons in the brain.

• Tissue-specific processing of pro-glucagon by prohormone convertases determines the relative abundance of glucagon in pancreas versus GLP-1/GLP-2 in intestine.

• Glucagon signals through the GCGR, a Gs protein-coupled receptor that activates adenylate cyclase and increases cAMP, mediating metabolic effects. Related references: PMID:32193322

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region of human pro-glucagon (residues 1–100), which is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For IHC-P, consider antigen retrieval protocols optimized for peptide hormones; validate staining specificity using glucagon-positive pancreatic islets or intestinal L cells.
• In ELISA, the antibody can be used for capture or detection; evaluate performance with recombinant glucagon or pro-glucagon fragments, and verify against known standards.
• Given the precursor-product relationship, the antibody may detect both full-length pro-glucagon and mature glucagon; confirm specificity with appropriate controls (e.g., tissues with differential processing).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and researchers with a comprehensive portfolio of raw materials for metabolic and endocrine studies. In addition to this anti-glucagon monoclonal antibody, we offer validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and access to rare targets like GLP-1 and GLP-2. Let us streamline your supply chain from discovery to commercial IVD development.