Anti-GLS2 Polyclonal Antibody for WB, ELISA - Q9UI32

Core Product Specifications and Parameters

Biological Background: GLS2 Function and Localization

• GLS2, also known as glutaminase liver isoform or L-glutaminase, is a mitochondrial enzyme encoded by the GLS2 (GA) gene in humans.
• It plays a crucial role in glutamine catabolism by catalyzing the hydrolysis of L-glutamine to glutamate and alpha-ketoglutarate, thereby fueling the tricarboxylic acid cycle and promoting mitochondrial respiration and ATP generation.
• GLS2 enhances cellular antioxidant defense through the production of NADH and glutathione, contributing to the maintenance of redox homeostasis.
• Highly expressed in the liver, with additional expression in the brain and pancreas, GLS2 is notably downregulated in hepatocellular carcinomas, suggesting a potential tumor-suppressive function.
• The protein is localized to the mitochondrion and contains an ANK repeat domain, which may mediate protein-protein interactions.
• GLS2 is subject to post-translational modifications including acetylation and alternative splicing, which may regulate its activity and function.

Experimental Guidance and Technical Tips

• The immunogen sequence corresponds to a specific region within the C-terminal part of human GLS2. For Western blotting, a band around 66 kDa is expected in human, mouse, and rat liver or brain tissue lysates.
• For ELISA applications, consider using the antibody as a capture or detection reagent in combination with a suitable pair to detect GLS2 levels in serum or tissue extracts.
• Cross-reactivity with mouse and rat GLS2 enables translational research across species; however, validation in the relevant sample system is recommended.
• Since GLS2 is a mitochondrial protein, sample preparation should include mitochondrial enrichment or whole-cell lysates with appropriate lysis buffers to ensure solubilization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop supplier of IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. We offer this Anti-GLS2 Polyclonal Antibody alongside validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and rare target sourcing to accelerate your metabolic or oncology assay development.