Anti-GLRX3 Polyclonal Antibody for WB, IHC-P, ELISA - O76003

Core Product Specifications and Parameters

Biological Background: Glutaredoxin-3 Function and Localization

• Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins. Related references: PMID:26613676, PMID:27519415
• Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity).
• Required for hemoglobin maturation. Related references: PMID:23615448
• Does not possess thioredoxin activity due to the lack of a conserved catalytic motif.
• Localizes to the cytoplasm, cell cortex, and myofibril sarcomere Z-line, suggesting roles in cytoskeletal organization and muscle contractility.
• Highly expressed in heart, spleen, testis; lower expression in thymus, leukocytes; weakly in lung, placenta, colon, small intestine.
• Known by alternative names PICOT (PKC-interacting cousin of thioredoxin) and TXNL2 (thioredoxin-like protein 2), indicating a structural relationship with thioredoxins.
• Features acetylation and phosphorylation modifications; contains iron-sulfur clusters and metal-binding sites essential for its Fe-S assembly function.

Experimental Guidance and Technical Tips

• The immunogen spans near-full-length human GLRX3 (aa 1-335), suggesting the polyclonal antibody recognizes multiple epitopes and may detect both unmodified and modified forms.
• For WB, consider using a reducing SDS-PAGE with DTT or β-mercaptoethanol and load 20–30 µg of cell or tissue lysate; the expected band is ~37 kDa.
• For IHC-P, antigen retrieval with citrate buffer (pH 6.0) or EDTA (pH 9.0) may enhance detection; include cardiac or splenic tissue as positive controls.
• For ELISA, the recombinant immunogen can serve as a positive control; validate cross-reactivity with mouse if using mouse-derived samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including validated antibodies for targets like GLRX3 involved in iron-sulfur cluster biology and cardiac function. Whether you need bulk ancillary reagents, optimized monoclonal/polyclonal antibodies, or rare-target sourcing support, our comprehensive supply chain bridges the gap from concept to clinic.