Anti-GLRX Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P35754

Core Product Specifications and Parameters

Biological Background: Glutaredoxin-1 Function and Localization

• Glutaredoxin-1 (GLRX), also known as thioltransferase-1 (TTase-1), is a small redox protein encoded by the GLRX gene.
• It possesses glutathione-disulfide oxidoreductase activity, catalyzing the reduction of disulfide bonds in the presence of NADPH and glutathione reductase.
• GLRX reduces low molecular weight disulfides and participates in the reduction of protein disulfides, playing a role in cellular redox homeostasis and electron transport.
• The protein is primarily localized in the cytoplasm, where it exerts its thiol-disulfide exchange function.
• GLRX contains a redox-active center with a conserved active site motif, essential for its enzymatic activity, and undergoes post-translational modification by acetylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length protein (aa 1-106), making this antibody suitable for detecting total GLRX in various applications.
• For Western blotting, a 12 kDa band is expected; consider using positive controls such as HeLa or HEK293 cell lysates, with validation under reducing conditions.
• In immunohistochemistry and immunofluorescence, optimize antigen retrieval and titrate the antibody to minimize background, as staining patterns may vary across tissues.
• For ELISA, the polyclonal nature can provide robust signal, but users should assess cross-reactivity with other glutaredoxin family members (e.g., GLRX2) if specificity is critical.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by offering streamlined access to validated IVD raw materials like this anti-GLRX polyclonal antibody, which serves as a reliable tool for redox biology and oxidative stress applications. Whether you need bulk quantities of optimized antibodies, ancillary reagents, or help sourcing rare targets, our comprehensive supply model can accelerate your development pipeline. Partner with CamelBio to simplify procurement and keep your projects moving from concept to clinic.