Anti-GLP2R Polyclonal Antibody for WB, IHC-P, ELISA - O95838

Core Product Specifications and Parameters

Biological Background: GLP2R Function and Localization

• GLP2R is a receptor for glucagon-like peptide 2 (GLP-2), a peptide hormone involved in regulating intestinal growth, nutrient absorption, and barrier function.
• The receptor belongs to the class B G protein-coupled receptor (GPCR) family and signals primarily through Gs proteins to activate adenylyl cyclase, leading to elevated intracellular cAMP levels.
• Encoded by the GLP2R gene on human chromosome 17, the protein comprises 553 amino acids with a predicted molecular weight of approximately 63 kDa.
• As a transmembrane receptor, GLP2R is predominantly localized at the cell membrane, facilitating its role in extracellular signal transduction.
• Key structural features include an N-terminal extracellular ligand-binding domain, seven transmembrane helices, and an intracellular C-terminal tail critical for G protein coupling and signaling.
• Post-translational modifications such as disulfide bond formation and glycosylation may contribute to receptor stability, folding, and membrane trafficking.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 460–553, the C-terminal intracellular domain, which may facilitate detection of the receptor in whole-cell lysates and membrane preparations.
• For Western blotting, expect a band near 63 kDa; consider using membrane protein enrichment and appropriate gel conditions to resolve the hydrophobic receptor.
• In IHC-P applications, antigen retrieval steps may be required; validate staining patterns in relevant human or mouse intestinal tissues, where GLP2R expression is expected.
• For ELISA, the antibody can be explored as a capture or detection reagent in sandwich formats when paired with a suitable clone or antibody pair.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw materials, offering validated antibodies, optimized polyclonal and monoclonal reagents, and bulk ancillary supplies. Our anti-GLP2R polyclonal antibody supports research into gastrointestinal hormone signaling and related diseases. With comprehensive sourcing capabilities, we help diagnostic manufacturers accelerate development from concept to clinic.