Anti-GLP1R Polyclonal Antibody for WB, IF/ICC, ELISA - P43220

Core Product Specifications and Parameters

Biological Background: GLP1R Function and Localization

• GLP1R functions as the receptor for glucagon-like peptide 1 (GLP-1), a key incretin hormone. Related references: PMID:19861722, PMID:26308095, PMID:27196125
• Ligand binding activates Gs protein-mediated adenylyl cyclase, increasing intracellular cAMP levels. Related references: PMID:19861722, PMID:26308095, PMID:27196125
• GLP1R plays a critical role in glucose homeostasis by regulating insulin secretion in response to GLP-1.
• The receptor is a multi-pass transmembrane protein localized predominantly to the cell membrane.
• GLP1R contains characteristic structural features of class B GPCRs, including an extracellular N-terminal domain, seven transmembrane helices, disulfide bonds, and N-linked glycosylation sites.
• The molecular weight of the unglycosylated receptor is approximately 53 kDa, with observed migration potentially affected by post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-140) corresponds to the N-terminal extracellular domain of human GLP1R, a region likely accessible for detecting native cell surface receptor in IF/ICC and ELISA applications.
• For Western blot, expect a band near 53 kDa; however, post-translational modifications such as N-linked glycosylation may cause migration shifts, so validate the observed band in the relevant sample system using appropriate controls.
• As a rabbit polyclonal antibody, it may exhibit lot-specific variation; including a positive control (e.g., GLP1R-overexpressing cell lysate) is recommended.
• Given cross-reactivity with mouse and rat, the antibody can be considered for translational studies in rodent models, but optimal dilution and assay conditions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering a comprehensive portfolio of GLP1R antibodies and associated reagents. From validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents, we support diagnostic developers targeting metabolic biomarkers. Our sourcing expertise also covers rare target raw materials, accelerating your assay development from concept to clinic.