Anti-GLP1R Polyclonal Antibody for WB and ELISA - P43220

Core Product Specifications and Parameters

Biological Background: GLP1R Function and Localization

• GLP1R, also known as GLP-1 receptor, is a G protein-coupled receptor encoded by the GLP1R gene in humans (Gene ID: 2740).
• Upon binding its ligand glucagon-like peptide 1 (GLP-1), GLP1R activates adenylyl cyclase, leading to increased intracellular cAMP levels. Related references: PMID:19861722, PMID:26308095, PMID:27196125
• This signaling cascade plays a critical role in regulating insulin secretion in response to GLP-1. Related references: PMID:19861722, PMID:26308095, PMID:27196125
• GLP1R is localized to the cell membrane and predicted to contain seven transmembrane domains.
• The receptor undergoes post-translational modifications including glycosylation and disulfide bond formation, which are important for proper folding and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (aa 22-116), which is involved in ligand binding; antibodies may affect receptor activation and are suitable for detecting native and denatured forms in techniques such as WB and ELISA.
• Given the receptor's molecular weight of ~53 kDa, process samples under reducing conditions and consider the possible impact of glycosylation on band migration.
• For species cross-reactivity, the antibody has been shown to react with human and rat GLP1R; verify reactivity in other species experimentally.
• For functional studies, consider using this antibody in neutralization assays or for receptor detection in pancreatic islets or GLP1R-overexpressing cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers from concept to clinic. This anti-GLP1R polyclonal antibody is part of our extensive portfolio for metabolic and endocrine research, and we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Contact us for custom material sourcing or technical consultation to accelerate your diagnostic development.