Anti-GLP-2 Rabbit Monoclonal Antibody for IF-P, IHC-P, ELISA - P01275

Core Product Specifications and Parameters

Biological Background: GLP-2 Function and Localization

• GLP-2 is a 33-amino acid peptide hormone derived from the pro-glucagon precursor (GCG gene) through tissue-specific proteolytic processing. It belongs to the glucagon family, which also includes glucagon, GLP-1, and oxyntomodulin.
• Plays a critical role in intestinal growth and maintenance by stimulating crypt cell proliferation and inhibiting enterocyte apoptosis, leading to increased villus height and enhanced nutrient absorption.
• Modulates gastrointestinal motility and secretion, including inhibition of gastric emptying and modulation of gastric acid secretion, which may contribute to satiety by causing a sensation of fullness.
• Key regulator of nutrient homeostasis: stimulates intestinal glucose transport and decreases mucosal permeability, enhancing nutrient assimilation and disposal.
• Secreted from enteroendocrine L cells predominantly located in the distal small intestine and colon; also expressed in selected neurons in the brain.
• Like other pro-glucagon-derived peptides, GLP-2 is subject to amidation and proteolytic processing at paired basic residues.
• May have a growth-promoting role in the neonatal intestine, supporting mucosal development during early life.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature GLP-2 sequence (amino acids 81–180 of pro-glucagon), ensuring specific recognition of GLP-2 over other pro-glucagon-derived peptides.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval may be required due to the small size of the peptide. Consider optimization using known positive control tissues such as intestinal epithelium.
• In ELISA applications, the antibody can be used as a capture or detection reagent; pairing with a compatible anti-GLP-2 antibody may enhance sensitivity. Validate performance in the relevant sample system.
• For immunofluorescence (IF-P), ensure adequate permeabilization to detect the secreted peptide.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents for gastrointestinal hormone research. This GLP-2 rabbit monoclonal antibody supports studies in metabolic disorders, intestinal biology, and related diagnostic development. As a one-stop partner, we streamline procurement from concept to clinic, ensuring access to quality-assured materials and technical support to accelerate your project timelines.