Anti-GLP-1 Monoclonal Antibody for IF-P, IHC-P, ELISA - P01275

Core Product Specifications and Parameters

Biological Background: GLP-1 Function and Localization

• GLP-1 (glucagon-like peptide-1) is a cleavage product of pro-glucagon, a hormone precursor encoded by the GCG gene, and acts as a pivotal incretin hormone in glucose homeostasis.
• It potently stimulates glucose-dependent insulin secretion from pancreatic beta cells and enhances insulin release in response to IL-6. Related references: PMID:22037645 PMID:40446798
• GLP-1 plays key roles in gastric motility and the suppression of plasma glucagon levels, contributing to overall metabolic control. Related references: PMID:10605628 PMID:14719035 PMID:12554744
• It suppresses satiety and stimulates glucose disposal in peripheral tissues independently of insulin, and significantly reduces food intake by inhibiting gastric emptying. Related references: PMID:10605628 PMID:14719035 PMID:12554744
• GLP-1 increases islet mass by promoting beta cell proliferation and inhibiting beta cell apoptosis, highlighting its therapeutic potential in diabetes. Related references: PMID:10605628 PMID:14719035 PMID:12554744
• It may regulate the hypothalamic-pituitary axis and influence secretion of LH, TSH, CRH, oxytocin, and vasopressin, indicating broader neuroendocrine roles. Related references: PMID:10605628 PMID:14719035 PMID:12554744
• Secreted predominantly from enteroendocrine L cells throughout the gastrointestinal tract and select neurons in the brain, GLP-1 exerts both peripheral and central effects. Related references: PMID:22037645

Experimental Guidance and Technical Tips

• The immunogen encompasses a region covering the bioactive GLP-1 sequence, ensuring targeted recognition; consider epitope exposure during assay development.
• For IF-P and IHC-P, optimized antigen retrieval and tissue fixation protocols may be necessary to preserve GLP-1 epitopes in pancreatic and intestinal samples.
• When incorporating this antibody into ELISA workflows, validate it against relevant biological matrices and consider peptide blocking to confirm specificity.
• Cross-reactivity with mouse and rat allows translational studies in these species, but species-specific expression differences should be accounted for in experimental designs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for seamless integration into diagnostic assays. For researchers focused on metabolic disorders such as diabetes and obesity, this anti-GLP-1 monoclonal antibody is a reliable tool, complemented by CamelBio’s capability to source rare targets and provide full-process technical support from concept to clinic.