Anti-GLI2 Polyclonal Antibody for WB, ELISA - P10070

Core Product Specifications and Parameters

Biological Background: GLI2 Function and Localization

• GLI2 is a zinc-finger transcription factor of the GLI family that serves as a key effector of the hedgehog signaling pathway, playing a critical role in skeleton development. Related references: PMID:15994174 PMID:18455992 PMID:19878745
• As a transcriptional activator, GLI2 binds to the GLI-consensus sequence 5'-GACCACCCA-3' and activates target gene expression upon smoothened (SMO) stimulation. Related references: PMID:18455992 PMID:19878745 PMID:24311597
• A proteolytically processed repressor form (GLI2R) is generated in the absence of hedgehog signal, constituting a minor pool and inhibiting target gene transcription (By similarity).
• GLI2 localizes to the nucleus, cytoplasm, and cell projections including the cilium, which is essential for hedgehog signal transduction.
• GLI2 undergoes multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination, as indicated by keyword annotations.
• In HTLV-1-infected T-cells, GLI2 acts as a Tax-dependent transcriptional activator by binding the Tax-responsive element (TRE-2S) to augment viral enhancer activity. Related references: PMID:9557682
• GLI2 is expressed in breast cancers and in HTLV-1-infected T-cell lines, with isoform-specific expression patterns. Related references: PMID:26565916 PMID:9557682

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-175) of mouse GLI2; it may detect both activator and repressor forms. Consider verifying specific band patterns in your model system.
• For Western blot, optimize antibody dilution using lysates from mouse or rat tissues known to express GLI2.
• For ELISA, validate assay conditions with recombinant GLI2 protein or peptide antigens to ensure specific detection.
• Cross-reactivity is reported for mouse and rat; confirm species specificity if using other models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner offering a broad portfolio of antibodies and reagents covering targets like GLI2 involved in hedgehog signaling and development. Our one-stop supply chain includes validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and support for rare target sourcing, helping diagnostic manufacturers and research labs streamline their projects from concept to clinic.