Anti-GLI2 Monoclonal Antibody for IF/ICC, IF-P, IHC-P, ELISA - P10070

Core Product Specifications and Parameters

Biological Background: GLI2 Function and Localization

• GLI2, also known as GLI family zinc finger protein 2, is a transcription factor that contains multiple zinc finger domains, enabling sequence-specific DNA binding to the consensus motif 5'-GACCACCCA-3'.
• It is a key effector of the hedgehog signaling pathway, transducing signals from the smoothened (SMO) receptor to regulate gene expression essential for skeleton development.
  Related references: PMID:15994174, PMID:18455992, PMID:19878745
• GLI2 functions as both a transcriptional activator and repressor; the full-length form acts as an activator upon hedgehog pathway stimulation, while a truncated repressor form (GLI2R) is generated by proteolytic processing in the absence of SMO signaling.
• In the nucleus, GLI2 activates target gene transcription following SMO activation, contributing to developmental processes and cellular proliferation.
  Related references: PMID:18455992, PMID:19878745, PMID:24311597
• The protein localizes to the nucleus, cytoplasm, and primary cilia, where it participates in signal transduction and transcriptional regulation.
• GLI2 is subject to post-translational modifications including phosphorylation, acetylation, and isopeptide bond formation, which modulate its stability and activity.
• It is expressed in various tissues, including breast cancers and HTLV-1-infected T-cell lines, indicating roles in both normal development and disease.
• (Microbial infection) In the context of HTLV-1 infection, GLI2 acts as a transcriptional activator of the viral enhancer in a Tax-dependent manner, binding to the Tax-responsive element TRE-2S.
  Related references: PMID:9557682

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1100-1200 of human GLI2, located in the C-terminal region. This internal sequence may be suitable for detecting both full-length and truncated forms, depending on the isoform.
• For immunofluorescence (IF/ICC), consider using appropriate fixation and permeabilization protocols; GLI2 localizes to the nucleus, cytoplasm, and cilia, so include controls for these compartments.
• In IHC-P, antigen retrieval may be necessary; validate staining in tissues known to express GLI2 (e.g., breast cancer or developmental samples) and include a nuclear counterstain.
• The antibody shows cross reactivity with mouse and rat, allowing its use in common model organisms for hedgehog pathway studies. Validate specificity in each species with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials and technical support, covering every stage from concept to clinic. For hedgehog pathway research and GLI2-related studies, we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our supply network also enables rapid sourcing of rare-target raw materials to accelerate your assay development.