Anti-GLI1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P08151

Core Product Specifications and Parameters

Biological Background: GLI1 Function and Localization

• GLI1 acts as a key transcription activator in the Hedgehog signaling pathway, regulating craniofacial development, digit formation, and the development of the central nervous system and gastrointestinal tract (PubMed:10806483, PubMed:11238441, PubMed:19706761). Related references: PMID:10806483 PMID:11238441 PMID:19706761

• It binds to the DNA consensus sequence 5'-GACCACCCA-3' to regulate target gene expression, a function activated upon Hedgehog ligand binding and Smoothened signaling (PubMed:2105456, PubMed:24217340, PubMed:2832761). Related references: PMID:2105456 PMID:24217340 PMID:2832761

• Unlike GLI2 and GLI3, GLI1 lacks a repressor domain and functions solely as a transcriptional activator (PubMed:10075717). Related references: PMID:10075717

• Two protein isoforms are produced by alternative splicing; isoform 1 is broadly expressed in normal tissues, whereas isoform 2 is undetectable in those tissues and exhibits a different target gene activation profile, including CD24 (PubMed:19706761). Related references: PMID:19706761

• Subcellular localization is dynamically regulated: GLI1 shuttles between the cytoplasm and nucleus and also localizes to primary cilia, which are critical for Hedgehog signal transduction.

• GLI1 is frequently overexpressed in cancers (e.g., glioma, basal cell carcinoma) and is regarded as a proto-oncogene, with its activity regulated by phosphorylation, acetylation, and ubiquitination.

• In normal physiology, GLI1 expression is highest in testis, myometrium, fallopian tube, and specific brain regions such as the cerebellum and olfactory tract.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 350–450 of human GLI1 (NP_001153517.1); this region lies within the central part of the protein and may be suitable for detecting both isoforms, but isoform-specific validation is recommended.
• This polyclonal antibody is suitable for Western blot (target band ~118 kDa), immunohistochemistry (paraffin), immunofluorescence/ICC, and ELISA. Optimize antibody dilution and blocking conditions for your specific sample type and assay format.
• For IHC-P, consider antigen retrieval methods (e.g., heat-induced epitope retrieval in citrate buffer) and include appropriate positive controls such as human cancer tissue sections known to overexpress GLI1.
• Cross-reactivity has been observed with mouse and rat samples; sequence homology suggests possible detection in these species, but confirmatory testing is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a streamlined supply of high-quality IVD raw materials, including this anti-GLI1 polyclonal antibody for studying Hedgehog pathway targets in development and oncology. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal reagents, and ancillary assay components, supported by technical consulting to accelerate your diagnostic development from concept to clinic.