Anti-GLI1 Rabbit Polyclonal Antibody for WB and ELISA - P08151

Core Product Specifications and Parameters

Biological Background: GLI1 Function and Localization

• GLI1 functions as a transcription activator and is a key effector of the Hedgehog (Hh) signaling pathway, playing essential roles in craniofacial development, digit formation, and central nervous system and gastrointestinal tract development. Related references: PMID:10806483 PMID:11238441 PMID:19706761

• It binds to the DNA consensus sequence 5'-GACCACCCA-3' in the promoters of target genes to modulate their expression. Related references: PMID:2105456 PMID:24217340 PMID:2832761

• Unlike the related transcription factors GLI2 and GLI3, GLI1 lacks a repressor domain and exclusively acts as a transcriptional activator. Related references: PMID:10075717

• Alternative splicing generates an isoform (isoform 2) that activates a distinct set of genes, including CD24, and is differentially expressed across tissues. Related references: PMID:19706761

• The protein localizes to the cytoplasm and nucleus, and is also found in the primary cilium, a signaling hub for the Hh pathway.

• Tissue expression is detected in testis, myometrium, fallopian tube, and specific brain regions such as the cerebellum, optic nerve, and olfactory tract; isoform 1 is broadly expressed while isoform 2 is more restricted.

• GLI1 is typically undetectable in normal adult cells but is highly upregulated in various cancers, where it functions as a proto-oncogene.

• The protein contains zinc-finger domains and is subject to post-translational modifications including phosphorylation, acetylation, and ubiquitination.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 118 kDa; use a suitable gel concentration and transfer conditions for high-molecular-weight proteins.
• Confirm antibody specificity in your experimental setup using positive (e.g., GLI1-overexpressing cells) and negative (e.g., GLI1-knockdown) controls.
• Given its cross-reactivity with mouse and rat, this antibody may be applied in rodent model studies.
• In ELISA, optimize the coating antigen concentration and antibody dilution to achieve the best signal-to-noise ratio.
• Note that GLI1 expression is often low in normal tissues; consider using cancer cell lines or tissues with active Hedgehog signaling for robust detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research institutions, offering one-stop access to validated IVD raw materials, bulk ancillary reagents, and custom antibody development. This anti-GLI1 polyclonal antibody supports research in developmental biology, oncology, and Hedgehog pathway signaling. Contact us to explore supply solutions tailored to your diagnostic assay development and reagent scaling needs.