Anti-GLE1 Polyclonal Antibody for WB, ELISA - Q53GS7

Core Product Specifications and Parameters

Biological Background: GLE1 Function and Localization

• The GLE1 (GLE1 RNA export mediator) gene encodes the mRNA export factor GLE1, also known as GLE1-like protein or Nucleoporin GLE1, which undergoes alternative splicing to generate multiple isoforms (UniProt: Q53GS7).
• GLE1 is required for the export of polyadenylated mRNAs from the nucleus to the cytoplasm, likely participating in the terminal translocation step through the nuclear pore complex (NPC).
• As a nucleoporin, GLE1 localizes primarily to the nuclear pore complex, shuttling between the nucleus and cytoplasm to facilitate mRNA transport.
• The protein contains a coiled-coil domain and is regulated by phosphorylation (keywords: Phosphoprotein).
• Mutations in GLE1 are associated with disease variants, including lethal congenital contracture syndrome (LCCS1) and related disorders (keywords: Disease variant; Alt Names include LCCS, LCCS1, CAAHC, CAAHD).
• GLE1 is implicated in protein transport and translocation processes across the nuclear envelope.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (aa 1-300) of human GLE1; epitopes may recognize multiple isoforms, and detection of bands above or below 80 kDa should be verified in the relevant sample system.
• For Western blot, consider using whole-cell lysates from human cell lines, as GLE1 is expressed in multiple tissues; a band corresponding to ~80 kDa is expected.
• In ELISA applications, the recombinant immunogen fragment can serve as a positive control to assess antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing everything from validated antibody pairs and optimized polyclonal antibodies (like this anti-GLE1 antibody) to bulk ancillary reagents and rare target raw-material sourcing. Whether you're developing assays for mRNA export pathway research or nuclear pore complex targets, our integrated supply chain supports your transition from concept to clinic with reliable, high-quality materials.