Anti-GLDC Rabbit PolymAb® for WB, IHC-P, IF/ICC, ELISA - P23378

Core Product Specifications and Parameters

Biological Background: Glycine dehydrogenase (decarboxylating), mitochondrial Function and Localization

• The protein is also known as Glycine cleavage system P protein, Glycine decarboxylase, or Glycine dehydrogenase (aminomethyl-transferring), encoded by the GLDC gene (also designated GCSP).
• It catalyzes the degradation of glycine by binding the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO₂ is released and the remaining methylamine moiety is transferred to the lipoamide cofactor of the H protein (GCSH).
• GLDC is a key component of the glycine cleavage system, which is essential for the metabolism of glycine, a major amino acid involved in various metabolic pathways.
• It is localized in the mitochondrion, indicating its role in mitochondrial energy metabolism.
• The protein belongs to the class of oxidoreductases and requires pyridoxal phosphate as a cofactor.
• Post-translational modifications such as acetylation have been identified through proteomics.
• Disease-associated variants have been reported for GLDC.
• The molecular weight of the mature protein is approximately 113 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 580–749, a C-terminal fragment; the antibody may recognize epitopes within this region.
• For Western blotting, a band around 113 kDa is expected; consider appropriate gel percentages and transfer conditions for high molecular weight proteins.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), mitochondrial localization may result in a punctate cytoplasmic staining pattern; validate in the relevant sample system.
• The antibody may be suitable for ELISA-based assays; users should optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials. For targets like glycine dehydrogenase (GLDC), we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our one-stop sourcing capability also covers rare target raw materials, all backed by technical expertise to streamline your development from concept to clinic.