Anti-GLDC Rabbit Polyclonal Antibody for WB, IF, IHC, ELISA - P23378

Core Product Specifications and Parameters

Biological Background: GLDC Function and Localization

• GLDC (glycine decarboxylase) is the P protein component of the mitochondrial glycine cleavage system, which catalyzes the degradation of glycine.
• It employs a pyridoxal phosphate cofactor to bind the alpha-amino group of glycine, releasing CO2 and transferring the remaining methylamine moiety to the H protein (GCSH).
• Also known as glycine dehydrogenase (decarboxylating) or glycine cleavage system P protein; aliases include GCE, GCSP, and HYGN1.
• The protein localizes to the mitochondrion, matching its role in amino acid catabolism.
• Post-translational modifications include acetylation, and variants are associated with disease (nonketotic hyperglycinemia).
• The molecular weight is approximately 112 kDa, and it is classified as an oxidoreductase with pyridoxal phosphate binding.
• It belongs to the reference proteome and has a 3D-structure determined, facilitating structural studies.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band near 112 kDa; validate with appropriate lysates from human, mouse, or rat tissues/cells.
• In immunohistochemistry (IHC-P), optimize antigen retrieval for formalin-fixed paraffin-embedded sections, using control tissues such as liver or brain that express GLDC.
• For immunofluorescence (IF), consider mitochondria-targeted co-stains to confirm localization, and optimize fixation/permeabilization to preserve mitochondrial integrity.
• The immunogen (aa 580-749, C-terminal) may not detect N-terminal truncations or specific isoforms; verify specificity in your system with knockdown/knockout controls if possible.
• Polyclonal antibodies may exhibit lot-specific variations; include positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a reliable provider of IVD raw materials, offering this anti-GLDC antibody to support glycine cleavage system and mitochondrial disorder research. Our sourcing network delivers ready-to-use validated antibodies, customized bulk reagents, and rare target procurement services to accelerate your assay development. Partner with CamelBio for seamless access to the reagents you need from concept to clinic.